Two Activation Steps of Carp Complement Requiring Ca2+and Mg2+and an Intermediate Product in Immune Hemolvsis

Tomoki Yano, Hiroko Ando, Miki Nakao

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

The action of carp complement on sensitized cells (EA) proceeds in two steps requiring Ca2+and Mg2+, respectively. In order to decide which of these steps precedes in immune hemolysis, we carried out a kinetic experiment by use of a buffer and carp serum depleted of cations by passage through Amberlite IRC-50. In a reaction mixture composed of EA (sheep red blood cells sensitized with carp antibody), carp complement, optimal Ca2+and optimal Mg2+, the hemolysis took place after a lag period of about 10 min, but when Ca2+was present at zero-time and Mg2+was added 20 min later, there was almost no lag. On the contrary, when Mg2+was present at zero-time and Ca2+was added 20 min later, there was a lag of about 20 min. These observations indicate that the step requiring Ca2+precedes that in which Mg2+functions. A hemolytic intermediate complex, which was provisionally designated EACx’, was prepared by treating EA with carp complement in the presence of Ca2+, but with the absence of Mg2+at 20°C for 10 min. Furthermore, carp serum free from Cx’, which was provisionally designated Cy’, was also obtained by treating resin-passed carp serum with excessive EA in the presence of Ca2+but absence of Mg2+at 0°C for 3 h. It was confirmed that the cells in the state EACx’ were completely lysed when treated with a sufficient quantity of Cy’ and optimal Mg2+, and that Cy’ did not react with EA even in the presence of optimal Mg2+.

元の言語英語
ページ(範囲)841-846
ページ数6
ジャーナルNippon Suisan Gakkaishi
51
発行部数5
DOI
出版物ステータス出版済み - 1 1 1985

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carp
serum
complement
calcium
sheep
hemolysis
antibody
resin
cation
blood
kinetics
intermediate product
resins
experiment
cations
erythrocytes
buffers
cells
antibodies

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Two Activation Steps of Carp Complement Requiring Ca2+and Mg2+and an Intermediate Product in Immune Hemolvsis. / Yano, Tomoki; Ando, Hiroko; Nakao, Miki.

:: Nippon Suisan Gakkaishi, 巻 51, 番号 5, 01.01.1985, p. 841-846.

研究成果: ジャーナルへの寄稿記事

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abstract = "The action of carp complement on sensitized cells (EA) proceeds in two steps requiring Ca2+and Mg2+, respectively. In order to decide which of these steps precedes in immune hemolysis, we carried out a kinetic experiment by use of a buffer and carp serum depleted of cations by passage through Amberlite IRC-50. In a reaction mixture composed of EA (sheep red blood cells sensitized with carp antibody), carp complement, optimal Ca2+and optimal Mg2+, the hemolysis took place after a lag period of about 10 min, but when Ca2+was present at zero-time and Mg2+was added 20 min later, there was almost no lag. On the contrary, when Mg2+was present at zero-time and Ca2+was added 20 min later, there was a lag of about 20 min. These observations indicate that the step requiring Ca2+precedes that in which Mg2+functions. A hemolytic intermediate complex, which was provisionally designated EACx’, was prepared by treating EA with carp complement in the presence of Ca2+, but with the absence of Mg2+at 20°C for 10 min. Furthermore, carp serum free from Cx’, which was provisionally designated Cy’, was also obtained by treating resin-passed carp serum with excessive EA in the presence of Ca2+but absence of Mg2+at 0°C for 3 h. It was confirmed that the cells in the state EACx’ were completely lysed when treated with a sufficient quantity of Cy’ and optimal Mg2+, and that Cy’ did not react with EA even in the presence of optimal Mg2+.",
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AB - The action of carp complement on sensitized cells (EA) proceeds in two steps requiring Ca2+and Mg2+, respectively. In order to decide which of these steps precedes in immune hemolysis, we carried out a kinetic experiment by use of a buffer and carp serum depleted of cations by passage through Amberlite IRC-50. In a reaction mixture composed of EA (sheep red blood cells sensitized with carp antibody), carp complement, optimal Ca2+and optimal Mg2+, the hemolysis took place after a lag period of about 10 min, but when Ca2+was present at zero-time and Mg2+was added 20 min later, there was almost no lag. On the contrary, when Mg2+was present at zero-time and Ca2+was added 20 min later, there was a lag of about 20 min. These observations indicate that the step requiring Ca2+precedes that in which Mg2+functions. A hemolytic intermediate complex, which was provisionally designated EACx’, was prepared by treating EA with carp complement in the presence of Ca2+, but with the absence of Mg2+at 20°C for 10 min. Furthermore, carp serum free from Cx’, which was provisionally designated Cy’, was also obtained by treating resin-passed carp serum with excessive EA in the presence of Ca2+but absence of Mg2+at 0°C for 3 h. It was confirmed that the cells in the state EACx’ were completely lysed when treated with a sufficient quantity of Cy’ and optimal Mg2+, and that Cy’ did not react with EA even in the presence of optimal Mg2+.

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