Two diverged complement factor B/C2-like cDNA sequences from a teleost, the common carp (Cyprinus carpio)

Miki Nakao, Yuko Fushitani, Kazuhiro Fujiki, Masaru Nonaka, Tomoki Yano

研究成果: ジャーナルへの寄稿学術誌査読

59 被引用数 (Scopus)

抄録

Mammalian complement components factor B and C2 act as proteolytic subunits of the C3 convertases in the alternative and the classical activation pathways, respectively, and are believed to have diverged from a common ancestor by gene duplication. However, it is unclear when the B/C2 duplication occurred. Here, we describe two diverged B/C2-like cDNA clones (B/C2-A and B/C2-B) isolated from a bony fish, the common carp (Cyprinus carpio). B/C2-A shares the same domain structure as the factor B and C2 complement components of vertebrates reported so far and shows a close similarity to zebrafish B and medaka fish B/C2. These teleost sequences show almost the same degree of similarity to C2 and B of higher vertebrates. In contrast, B/C2-B has a novel structural feature in thai it contains four short consensus repeat modules and does not have a close relative upon phylogenetic analysis. Northern blotting revealed the presence of two transcripts with different sizes for both the B/C2-A and B/C2-B in the hepatopancreas of the carp. Southern blotting suggested the presence of multiple genes for B/C2-A and a single gene for B/C2-B. Although structural features of B/C2-B are slightly more C2-like than B-like, B/C2-B has a crucial amino acid substitution in the serine protease domain, which makes it unlikely that B/C2-B functions as a C3 convertase. A possible phylogenetic relationship between the two carp sequences and mammalian C2 and B is discussed.

本文言語英語
ページ(範囲)4811-4818
ページ数8
ジャーナルJournal of Immunology
161
9
出版ステータス出版済み - 11月 1 1998

!!!All Science Journal Classification (ASJC) codes

  • 免疫学

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