Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis

Hideo Nishitani, Nozomi Sugimoto, Vassilis Roukos, Yohsuke Nakanishi, Masafumi Saijo, Chikashi Obuse, Toshiki Tsurimoto, Keiichi I. Nakayama, Keiko Nakayama, Masatoshi Fujita, Zoi Lygerou, Takeharu Nishimoto

研究成果: ジャーナルへの寄稿記事

276 引用 (Scopus)

抄録

Replication licensing is carefully regulated to restrict replication to once in a cell cycle. In higher eukaryotes, regulation of the licensing factor Cdt1 by proteolysis and Geminin is essential to prevent re-replication. We show here that the N-terminal 100 amino acids of human Cdt1 are recognized for proteolysis by two distinct E3 ubiquitin ligases during S-G2 phases. Six highly conserved amino acids within the 10 first amino acids of Cdt1 are essential for DDB1-Cul4-mediated proteolysis. This region is also involved in proteolysis following DNA damage. The second E3 is SCF-Skp2, which recognizes the Cy-motif-mediated Cyclin E/A-cyclin-dependent kinase-phosphorylated region. Consistently, in HeLa cells cosilenced of Skp2 and Cul4, Cdt1 remained stable in S-G2 phases. The Cul4-containing E3 is active during ongoing replication, while SCF-Skp2 operates both in S and G2 phases. PCNA binds to Cdt1 through the six conserved N-terminal amino acids. PCNA is essential for Cul4- but not Skp2-directed degradation during DNA replication and following ultraviolet-irradiation. Our data unravel multiple distinct pathways regulating Cdt1 to block re-replication.

元の言語英語
ページ(範囲)1126-1136
ページ数11
ジャーナルEMBO Journal
25
発行部数5
DOI
出版物ステータス出版済み - 3 8 2006

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Proteolysis
Ubiquitin-Protein Ligases
G2 Phase
S Phase
Amino Acids
Proliferating Cell Nuclear Antigen
Licensure
Geminin
Cyclin E
Essential Amino Acids
Cyclin-Dependent Kinases
DNA
Eukaryota
DNA Replication
HeLa Cells
DNA Damage
Cell Cycle
Cells
Irradiation
Degradation

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Nishitani, H., Sugimoto, N., Roukos, V., Nakanishi, Y., Saijo, M., Obuse, C., ... Nishimoto, T. (2006). Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. EMBO Journal, 25(5), 1126-1136. https://doi.org/10.1038/sj.emboj.7601002

Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. / Nishitani, Hideo; Sugimoto, Nozomi; Roukos, Vassilis; Nakanishi, Yohsuke; Saijo, Masafumi; Obuse, Chikashi; Tsurimoto, Toshiki; Nakayama, Keiichi I.; Nakayama, Keiko; Fujita, Masatoshi; Lygerou, Zoi; Nishimoto, Takeharu.

:: EMBO Journal, 巻 25, 番号 5, 08.03.2006, p. 1126-1136.

研究成果: ジャーナルへの寄稿記事

Nishitani, H, Sugimoto, N, Roukos, V, Nakanishi, Y, Saijo, M, Obuse, C, Tsurimoto, T, Nakayama, KI, Nakayama, K, Fujita, M, Lygerou, Z & Nishimoto, T 2006, 'Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis', EMBO Journal, 巻. 25, 番号 5, pp. 1126-1136. https://doi.org/10.1038/sj.emboj.7601002
Nishitani, Hideo ; Sugimoto, Nozomi ; Roukos, Vassilis ; Nakanishi, Yohsuke ; Saijo, Masafumi ; Obuse, Chikashi ; Tsurimoto, Toshiki ; Nakayama, Keiichi I. ; Nakayama, Keiko ; Fujita, Masatoshi ; Lygerou, Zoi ; Nishimoto, Takeharu. / Two E3 ubiquitin ligases, SCF-Skp2 and DDB1-Cul4, target human Cdt1 for proteolysis. :: EMBO Journal. 2006 ; 巻 25, 番号 5. pp. 1126-1136.
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abstract = "Replication licensing is carefully regulated to restrict replication to once in a cell cycle. In higher eukaryotes, regulation of the licensing factor Cdt1 by proteolysis and Geminin is essential to prevent re-replication. We show here that the N-terminal 100 amino acids of human Cdt1 are recognized for proteolysis by two distinct E3 ubiquitin ligases during S-G2 phases. Six highly conserved amino acids within the 10 first amino acids of Cdt1 are essential for DDB1-Cul4-mediated proteolysis. This region is also involved in proteolysis following DNA damage. The second E3 is SCF-Skp2, which recognizes the Cy-motif-mediated Cyclin E/A-cyclin-dependent kinase-phosphorylated region. Consistently, in HeLa cells cosilenced of Skp2 and Cul4, Cdt1 remained stable in S-G2 phases. The Cul4-containing E3 is active during ongoing replication, while SCF-Skp2 operates both in S and G2 phases. PCNA binds to Cdt1 through the six conserved N-terminal amino acids. PCNA is essential for Cul4- but not Skp2-directed degradation during DNA replication and following ultraviolet-irradiation. Our data unravel multiple distinct pathways regulating Cdt1 to block re-replication.",
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