Two low-temperature-inducible Chlorella genes for Δ12 and ω-3 Fatty Acid Desaturase (FAD): Isolation of Δ12 and ω-3 fad cDNA clones, expression of Δ12 fad in Saccharomyces cerevisiae, and expression of ω-3 fad in Nicotiana tabacum

Koushirou Suga, Ken-ichi Honjoh, Naoki Furuya, Hideyuki Shimizu, Koutarou Nishi, Fuminori Shinohara, Yoshie Hirabaru, Isao Maruyama, Takahisa Miyamoto, Shoji Hatano, Masayoshi Iio

研究成果: Contribution to journalArticle査読

41 被引用数 (Scopus)


In an attempt to clarify the involvement of fatty acid desaturases (FADs) in the freezing tolerance of Chlorella vulgaris IAM C-27, developed by hardening, we have isolated cDNA clones for two types of FADs from the Chlorella strain, based on the sequence information of genes for Δ12 and ω-3 FADs, respectively desaturating oleic acid (18:1) to linoleic acid (18:2) and linoleic acid (18:2) to linolenic acid (18:3). The deduced amino acid sequence of the first clone, designated CvFad2, showed about 66% similarity to the microsomal Δ12 FADs from several higher plants and this gene had Δ12 FAD activity when expressed in Saccharomyces cerevisiae. The predicted protein encoded by a second gene, designated CvFad3, showed about 60% similarity to the microsomal and plastidial ω-3 FADs from several higher plants. The features of the amino acid sequences of the C- and N-terminal regions of CvFAD3 and fatty acid analysis of polar lipids in transgenic tobacco plant expressing the CvFad3 gene suggested that this gene encodes the microsomal ω-3 FAD. Southern blot analysis showed that both genes were single-copy genes in the genome of the Chlorella strain. Different transcriptional patterns were observed with the two genes during hardening in Northern blot analysis.

ジャーナルBioscience, Biotechnology and Biochemistry
出版ステータス出版済み - 1 1 2002

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学


「Two low-temperature-inducible Chlorella genes for Δ12 and ω-3 Fatty Acid Desaturase (FAD): Isolation of Δ12 and ω-3 <i>fad</i> cDNA clones, expression of Δ12 <i>fad</i> in <i>Saccharomyces cerevisiae</i>, and expression of ω-3 <i>fad</i> in <i>Nicotiana tabacum</i>」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。