Two-step concerted mechanism for the hydrocarbon hydroxylation by cytochrome P450

Kazunari Yoshizawa, Takehiro Ohta, Masataka Eda, Tokio Yamabe

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

A possible two-step mechanism for the hydrocarbon hydroxylation by the oxoferryl active species of cytochrome P450 is discussed. We propose that the catalytic reaction is initiated by the formation of a reactant complex involving a five-coordinate carbon species or a α-complex, followed by concerted hydrogen and alkyl migrations which lead to the formation of a product complex involving alcohol as a ligand. The porphyrin ring is predicted from molecular mechanics and extended Huckel calculations to be significantly bent in the reactant complex and in the hydroxy intermediate to form an energetically preferred octahedral environment at the iron active center. Such bent structures for the porphyrin ring are consistent with observed cis-coordination geometries for the M(porphyrin)L2 complexes, in which M is a large transition- metal ion and L is a ligand. We suggest that possible inversion at the five-coordinate carbon species in the reactant complex can occur to lead to partial inversion of stereochemistry at a labeled carbon.

元の言語英語
ページ(範囲)401-407
ページ数7
ジャーナルBulletin of the Chemical Society of Japan
73
発行部数2
DOI
出版物ステータス出版済み - 2 1 2000
外部発表Yes

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Hydroxylation
Porphyrins
Hydrocarbons
Cytochrome P-450 Enzyme System
Carbon
Ligands
Stereochemistry
Molecular mechanics
Transition metals
Metal ions
Hydrogen
Iron
Alcohols
Geometry

All Science Journal Classification (ASJC) codes

  • Chemistry(all)

これを引用

Two-step concerted mechanism for the hydrocarbon hydroxylation by cytochrome P450. / Yoshizawa, Kazunari; Ohta, Takehiro; Eda, Masataka; Yamabe, Tokio.

:: Bulletin of the Chemical Society of Japan, 巻 73, 番号 2, 01.02.2000, p. 401-407.

研究成果: ジャーナルへの寄稿記事

Yoshizawa, Kazunari ; Ohta, Takehiro ; Eda, Masataka ; Yamabe, Tokio. / Two-step concerted mechanism for the hydrocarbon hydroxylation by cytochrome P450. :: Bulletin of the Chemical Society of Japan. 2000 ; 巻 73, 番号 2. pp. 401-407.
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