Two vitellogenins in the loliginid squid Uroteuthis edulis

Identification and specific expression in ovarian follicles

Hajime Kitano, Naoki Nagano, Keishi Sakaguchi, Michiya Matsuyama

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Vitellogenenesis is a physiological process common in oviparous animals. The molecular profile, modifications, and utilization of vitellogenin (VTG), a precursor of yolk protein, have been characterized in various taxa to understand oogenesis within different modes of reproduction. Hormonal regulation of VTGs has been investigated in invertebrates, such as insects and crustaceans; conversely, little is known for cephalopods. In this study, we isolated two VTG genes (ue-VTG1 and ue-VTG2) from the loliginid swordtip squid, Uroteuthis edulis, via a comprehensive survey of a transcriptome database and subsequent cDNA cloning. Structural analysis of the two ue-VTGs revealed their unique features, namely the absence of two domains usually found in VTGs from other organisms: the von Willebrand factor D domain (vWD) and the domain of unknown function 1943 (DUF1943). Levels of ue-VTG1 and ue-VTG2 transcripts in the ovary, specifically in follicular cells, increased during the late-vitellogenic phase, suggesting that yolk accumulation progresses via paracrine interactions involving follicular cells and oocytes. N-terminal amino acid sequencing of biochemically purified yolk protein revealed its origins from these two VTGs, indicating that both are functional precursors of yolk protein. These results provide information that is essential to understanding the physiological pathway of yolk synthesis, accumulation, and storage in loliginid squids.

元の言語英語
ページ(範囲)363-375
ページ数13
ジャーナルMolecular Reproduction and Development
84
発行部数5
DOI
出版物ステータス出版済み - 5 1 2017

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Vitellogenins
Egg Proteins
Decapodiformes
Ovarian Follicle
Oviparity
Cephalopoda
Physiological Phenomena
Oogenesis
Protein Sequence Analysis
von Willebrand Factor
Invertebrates
Transcriptome
Oocytes
Reproduction
Insects
Organism Cloning
Ovary
Complementary DNA
Databases
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology
  • Cell Biology

これを引用

Two vitellogenins in the loliginid squid Uroteuthis edulis : Identification and specific expression in ovarian follicles. / Kitano, Hajime; Nagano, Naoki; Sakaguchi, Keishi; Matsuyama, Michiya.

:: Molecular Reproduction and Development, 巻 84, 番号 5, 01.05.2017, p. 363-375.

研究成果: ジャーナルへの寄稿記事

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