U Box Proteins as a New Family of Ubiquitin-Protein Ligases

Shigetsugu Hatakeyama, Masayoshi Yada, Masaki Matsumoto, Noriko Ishida, Kei Ichi Nakayama

研究成果: ジャーナルへの寄稿記事

428 引用 (Scopus)

抄録

The U box is a domain of ∼70 amino acids that is present in proteins from yeast to humans. The prototype U box protein, yeast Ufd2, was identified as a ubiquitin chain assembly factor that cooperates with a ubiquitin-activating enzyme (El), a ubiquitin-conjugating enzyme (E2), and a ubiquitin-protein ligase (E3) to catalyze ubiquitin chain formation on artificial substrates. E3 enzymes are thought to determine the substrate specificity of ubiquitination and have been classified into two families, the HECT and RING finger families. Six mammalian U box proteins have now been shown to mediate polyubiquitination in the presence of E1 and E2 and in the absence of E3. These U box proteins exhibited different specificities for E2 enzymes in this reaction. Deletion of the U box or mutation of conserved amino acids within it abolished ubiquitination activity. Some U box proteins catalyzed polyubiquitination by targeting lysine residues of ubiquitin other than lysine 48, which is utilized by HECT and RING finger E3 enzymes for polyubiquitination that serves as a signal for proteolysis by the 26 S proteasome. These data suggest that U box proteins constitute a third family of E3 enzymes and that E4 activity may reflect a specialized type of E3 activity.

元の言語英語
ページ(範囲)33111-33120
ページ数10
ジャーナルJournal of Biological Chemistry
276
発行部数35
DOI
出版物ステータス出版済み - 8 31 2001

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Ubiquitin-Protein Ligases
Ubiquitin
Fungal Proteins
Ubiquitination
Enzymes
Lysine
Proteins
Ubiquitin-Activating Enzymes
Ubiquitin-Conjugating Enzymes
Proteolysis
Amino Acids
Proteasome Endopeptidase Complex
Substrates
Substrate Specificity
Mutation

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

U Box Proteins as a New Family of Ubiquitin-Protein Ligases. / Hatakeyama, Shigetsugu; Yada, Masayoshi; Matsumoto, Masaki; Ishida, Noriko; Nakayama, Kei Ichi.

:: Journal of Biological Chemistry, 巻 276, 番号 35, 31.08.2001, p. 33111-33120.

研究成果: ジャーナルへの寄稿記事

Hatakeyama, Shigetsugu ; Yada, Masayoshi ; Matsumoto, Masaki ; Ishida, Noriko ; Nakayama, Kei Ichi. / U Box Proteins as a New Family of Ubiquitin-Protein Ligases. :: Journal of Biological Chemistry. 2001 ; 巻 276, 番号 35. pp. 33111-33120.
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