抄録
The periodic destruction of RAG-2 at the G1-to-S transition couples V(D)J recombination to the G0 and G1 cell cycle phases and coordinates RAG-mediated DNA cleavage with DNA repair by nonhomologous end joining. To define the mechanism by which this occurs, we reproduced cell cycle-dependent regulation of the V(D)J recombinase in a cell-free system. The ubiquitin-proteasomal pathway carries out destruction of RAG-2 in lysates of S phase cells and during S phase in vivo. Remarkably, the Skp2-SCF ubiquitin ligase, which plays a central role in cell cycle regulation through the destruction of p27, mediates ubiquitylation of RAG-2 in vitro and degradation of RAG-2 in vivo. The regulation of antigen receptor gene assembly by Skp2-SCF provides an unexpected and direct mechanistic link between DNA recombination and the cell cycle.
本文言語 | 英語 |
---|---|
ページ(範囲) | 699-709 |
ページ数 | 11 |
ジャーナル | Molecular Cell |
巻 | 18 |
号 | 6 |
DOI | |
出版ステータス | 出版済み - 6月 10 2005 |
All Science Journal Classification (ASJC) codes
- 分子生物学
- 細胞生物学