抄録
The periodic destruction of RAG-2 at the G1-to-S transition couples V(D)J recombination to the G0 and G1 cell cycle phases and coordinates RAG-mediated DNA cleavage with DNA repair by nonhomologous end joining. To define the mechanism by which this occurs, we reproduced cell cycle-dependent regulation of the V(D)J recombinase in a cell-free system. The ubiquitin-proteasomal pathway carries out destruction of RAG-2 in lysates of S phase cells and during S phase in vivo. Remarkably, the Skp2-SCF ubiquitin ligase, which plays a central role in cell cycle regulation through the destruction of p27, mediates ubiquitylation of RAG-2 in vitro and degradation of RAG-2 in vivo. The regulation of antigen receptor gene assembly by Skp2-SCF provides an unexpected and direct mechanistic link between DNA recombination and the cell cycle.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 699-709 |
| ページ数 | 11 |
| ジャーナル | Molecular Cell |
| 巻 | 18 |
| 発行部数 | 6 |
| DOI | |
| 出版物ステータス | 出版済み - 6 10 2005 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell Biology
これを引用
Ubiquitylation of RAG-2 by Skp2-SCF links destruction of the V(D)J recombinase to the cell cycle. / Jiang, Hao; Chang, Fu Chung; Ross, Ashley E.; Lee, Jihyun; Nakayama, Keiichi; Nakayama, Keiko; Desiderio, Stephen.
：: Molecular Cell, 巻 18, 番号 6, 10.06.2005, p. 699-709.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Ubiquitylation of RAG-2 by Skp2-SCF links destruction of the V(D)J recombinase to the cell cycle
AU - Jiang, Hao
AU - Chang, Fu Chung
AU - Ross, Ashley E.
AU - Lee, Jihyun
AU - Nakayama, Keiichi
AU - Nakayama, Keiko
AU - Desiderio, Stephen
PY - 2005/6/10
Y1 - 2005/6/10
N2 - The periodic destruction of RAG-2 at the G1-to-S transition couples V(D)J recombination to the G0 and G1 cell cycle phases and coordinates RAG-mediated DNA cleavage with DNA repair by nonhomologous end joining. To define the mechanism by which this occurs, we reproduced cell cycle-dependent regulation of the V(D)J recombinase in a cell-free system. The ubiquitin-proteasomal pathway carries out destruction of RAG-2 in lysates of S phase cells and during S phase in vivo. Remarkably, the Skp2-SCF ubiquitin ligase, which plays a central role in cell cycle regulation through the destruction of p27, mediates ubiquitylation of RAG-2 in vitro and degradation of RAG-2 in vivo. The regulation of antigen receptor gene assembly by Skp2-SCF provides an unexpected and direct mechanistic link between DNA recombination and the cell cycle.
AB - The periodic destruction of RAG-2 at the G1-to-S transition couples V(D)J recombination to the G0 and G1 cell cycle phases and coordinates RAG-mediated DNA cleavage with DNA repair by nonhomologous end joining. To define the mechanism by which this occurs, we reproduced cell cycle-dependent regulation of the V(D)J recombinase in a cell-free system. The ubiquitin-proteasomal pathway carries out destruction of RAG-2 in lysates of S phase cells and during S phase in vivo. Remarkably, the Skp2-SCF ubiquitin ligase, which plays a central role in cell cycle regulation through the destruction of p27, mediates ubiquitylation of RAG-2 in vitro and degradation of RAG-2 in vivo. The regulation of antigen receptor gene assembly by Skp2-SCF provides an unexpected and direct mechanistic link between DNA recombination and the cell cycle.
UR - http://www.scopus.com/inward/record.url?scp=20444403003&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20444403003&partnerID=8YFLogxK
U2 - 10.1016/j.molcel.2005.05.011
DO - 10.1016/j.molcel.2005.05.011
M3 - Article
C2 - 15949444
AN - SCOPUS:20444403003
VL - 18
SP - 699
EP - 709
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 6
ER -