Ultra-Fast Electron Microscopic Imaging of Single Molecules with a Direct Electron Detection Camera and Noise Reduction

Joshua Stuckner, Toshiki Shimizu, Koji Harano, Eiichi Nakamura, Mitsuhiro Murayama

研究成果: Contribution to journalArticle査読

3 被引用数 (Scopus)

抄録

Abstract Time-resolved imaging of molecules and materials made of light elements is an emerging field of transmission electron microscopy (TEM), and the recent development of direct electron detection cameras, capable of taking as many as 1,600 fps, has potentially broadened the scope of the time-resolved TEM imaging in chemistry and nanotechnology. However, such a high frame rate reduces electron dose per frame, lowers the signal-to-noise ratio (SNR), and renders the molecular images practically invisible. Here, we examined image noise reduction to take the best advantage of fast cameras and concluded that the Chambolle total variation denoising algorithm is the method of choice, as illustrated for imaging of a molecule in the 1D hollow space of a carbon nanotube with ~1 ms time resolution. Through the systematic comparison of the performance of multiple denoising algorithms, we found that the Chambolle algorithm improves the SNR by more than an order of magnitude when applied to TEM images taken at a low electron dose as required for imaging at around 1,000 fps. Open-source code and a standalone application to apply Chambolle denoising to TEM images and video frames are available for download.

本文言語英語
ページ(範囲)667-675
ページ数9
ジャーナルMicroscopy and Microanalysis
26
4
DOI
出版ステータス出版済み - 8 1 2020
外部発表はい

All Science Journal Classification (ASJC) codes

  • 器械工学

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