Ultrathin sectioning with DUV-pulsed laser ablation: Development of a laser ablation nano tome

Takaaki Kanemaru, Yuji Oki

研究成果: ジャーナルへの寄稿レター

2 引用 (Scopus)

抄録

The electrically automated ultrathin sectioning apparatus, which has been developed in recent years, can produce consecutive ultrathin sections with a diamond knife and a gallium ion beam. These newly developed apparatuses, however, have several shortcomings, such as the limited block cutting area, thermal damage to the sample by the focused ion beam and a sample electronic charge. To overcome these faults and for easier scanning electron microscopy three-dimensional fine structural reconstruction, we have developed a new cutting method using a deep ultraviolet laser, which we have named the ‘LANTome (Light Ablation Nanotome)’. Using this method, we confirmed the widening of sectioning areas, shortening of the sectioning time, automatic smoothing of rough surfaces, no sample electronic charge and minimal heat effects on the sample tissue, such as thermal denaturation.

元の言語英語
ページ(範囲)289-296
ページ数8
ジャーナルMicroscopy
64
発行部数4
DOI
出版物ステータス出版済み - 8 1 2015

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Laser Therapy
Laser ablation
Pulsed lasers
laser ablation
pulsed lasers
Lasers
Hot Temperature
Ultraviolet lasers
Denaturation
Focused ion beams
Gallium
Ablation
Thermal effects
Ions
Ion beams
Diamonds
Diamond
ion beams
Tissue
Electron Scanning Microscopy

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Instrumentation
  • Radiology Nuclear Medicine and imaging

これを引用

Ultrathin sectioning with DUV-pulsed laser ablation : Development of a laser ablation nano tome. / Kanemaru, Takaaki; Oki, Yuji.

:: Microscopy, 巻 64, 番号 4, 01.08.2015, p. 289-296.

研究成果: ジャーナルへの寄稿レター

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