Ultrathin sectioning with DUV-pulsed laser ablation: Development of a laser ablation nano tome

Takaaki Kanemaru, Yuji Oki

研究成果: Contribution to journalLetter査読

2 被引用数 (Scopus)

抄録

The electrically automated ultrathin sectioning apparatus, which has been developed in recent years, can produce consecutive ultrathin sections with a diamond knife and a gallium ion beam. These newly developed apparatuses, however, have several shortcomings, such as the limited block cutting area, thermal damage to the sample by the focused ion beam and a sample electronic charge. To overcome these faults and for easier scanning electron microscopy three-dimensional fine structural reconstruction, we have developed a new cutting method using a deep ultraviolet laser, which we have named the ‘LANTome (Light Ablation Nanotome)’. Using this method, we confirmed the widening of sectioning areas, shortening of the sectioning time, automatic smoothing of rough surfaces, no sample electronic charge and minimal heat effects on the sample tissue, such as thermal denaturation.

本文言語英語
ページ(範囲)289-296
ページ数8
ジャーナルMicroscopy
64
4
DOI
出版ステータス出版済み - 8 1 2015

All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 器械工学
  • 放射線学、核医学およびイメージング

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