Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Hiroya Ishikawa; Mitsuya Shimoda; Akiyoshi Yonekura; Keiko Mishima; Kiyoshi Matsumoto; Yutaka Osajima

doi:10.1271/bbb.64.1295

Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Akiyoshi Yonekura, Keiko Mishima, Kiyoshi Matsumoto, Yutaka Osajima

食料化学工学

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

2 被引用数 (Scopus)

抄録

The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO₂ were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO₂. No important changes were observed in heating, autoclaving, or pH-lowering.

本文言語	英語
ページ（範囲）	1295-1297
ページ数	3
ジャーナル	Bioscience, Biotechnology and Biochemistry
巻	64
号	6
DOI	https://doi.org/10.1271/bbb.64.1295
出版ステータス	出版済み - 2000

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
分析化学
生化学
応用微生物学とバイオテクノロジー
分子生物学
有機化学

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10.1271/bbb.64.1295

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abstract = "The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.",

author = "Hiroya Ishikawa and Mitsuya Shimoda and Akiyoshi Yonekura and Keiko Mishima and Kiyoshi Matsumoto and Yutaka Osajima",

year = "2000",

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AU - Ishikawa, Hiroya

AU - Shimoda, Mitsuya

AU - Yonekura, Akiyoshi

AU - Mishima, Keiko

AU - Matsumoto, Kiyoshi

AU - Osajima, Yutaka

PY - 2000

Y1 - 2000

N2 - The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.

AB - The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.

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JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

SN - 0916-8451

IS - 6

ER -

Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

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!!!All Science Journal Classification (ASJC) codes

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