Unfolding of poly-L-glutamic acid by microbubbling of supercritical carbon dioxide

Hiroya Ishikawa, Mitsuya Shimoda, Akiyoshi Yonekura, Keiko Mishima, Kiyoshi Matsumoto, Yutaka Osajima

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

The conformational changes in α-helical poly-L-glutamic acid caused by microbubbling supercritical CO2 were investigated with circular dichroism spectra. After microbubbling using a micropore filter at 35 and 30 MPa for 30 min, α-helix content decreased to 37%, while without the filter it was 68%. The α-helix structure was significantly decomposed by a high density of CO2. No important changes were observed in heating, autoclaving, or pH-lowering.

本文言語英語
ページ(範囲)1295-1297
ページ数3
ジャーナルBioscience, Biotechnology and Biochemistry
64
6
DOI
出版ステータス出版済み - 2000

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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