Unique activation status of peripheral blood mononuclear cells at acute phase of Kawasaki disease

K. Ikeda, K. Yamaguchi, T. Tanaka, Y. Mizuno, A. Hijikata, O. Ohara, H. Takada, K. Kusuhara, T. Hara

研究成果: Contribution to journalArticle査読

51 被引用数 (Scopus)

抄録

Summary Although Kawasaki disease (KD) is characterized by a marked activation of the immune system with elevations of serum proinflammatory cytokines and chemokines at acute phase, the major sources for these chemical mediators remain controversial. We analysed the activation status of peripheral blood mononuclear cells (PBMCs) by flow cytometry, DNA microarray and quantitative reverse transcription-polymerase chain reaction. The proportions of CD69+ cells in both natural killer cells and γδT cells at acute-phase KD were significantly higher than those at convalescent-phase KD. Microarray analysis revealed that five genes such as NAIP, IPAF, S100A9, FCGR1A and GCA up-regulated in acute-phase KD and the pathways involved in acute phase KD were related closely to the innate immune system. The relative expression levels of damage-associated molecular pattern molecule (DAMP) (S100A9 and S100A12) genes in PBMCs at acute-phase KD were significantly higher than those at convalescent-phase KD, while those of TNFA, IL1B and IL6 genes were not significantly different between KD patients and healthy controls. Intracellular production of tumour necrosis factor-α, interlaukin-10 and interferon-γ in PBMCs was not observed in KD patients. The present data have indicated that PBMCs showed a unique activation status with high expression of DAMP genes but low expression of proinflammatory cytokine genes, and that the innate immune system appears to play a role in the pathogenesis and pathophysiology of KD.

本文言語英語
ページ(範囲)246-255
ページ数10
ジャーナルClinical and Experimental Immunology
160
2
DOI
出版ステータス出版済み - 5 2010

All Science Journal Classification (ASJC) codes

  • 免疫アレルギー学
  • 免疫学

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