Unique hyper-thermal composting process in Kagoshima City forms distinct bacterial community structures

Yukihiro Tashiro, Hanae Tabata, Asuka Itahara, Natsuki Shimizu, Kosuke Tashiro, Kenji Sakai

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

A unique compost, Satsuma soil, is produced from three types of wastewater sludge using hyper-thermal processes at temperatures much higher than that of general thermophilic processes in Kagoshima City, Japan. We analyzed the bacterial community structures of this hyper-thermal compost sample and other sludges and composts by a high-throughput barcoded pyrosequencing method targeting the 16S rRNA gene. In total, 621,076 reads were derived from 17 samples and filtered. Artificial sequences were deleted and the reads were clustered based on the operational taxonomic units (OTUs) at 97% similarity. Phylum-level analysis of the hyper-thermal compost revealed drastic changes of the sludge structures (each relative abundance) from Firmicutes (average 47.8%), Proteobacteria (average 22.3%), and Bacteroidetes (average 10.1%) to two main phyla including Firmicutes (73.6%) and Actinobacteria (25.0%) with less Proteobacteria (∼0.3%) and Bacteroidetes (∼0.1%). Furthermore, we determined the predominant species (each relative abundance) of the hyper-thermal compost including Firmicutes related to Staphylococcus cohnii (13.8%), Jeotgalicoccus coquinae (8.01%), and Staphylococcus lentus (5.96%), and Actinobacteria related to Corynebacterium stationis (6.41%), and found that these species were not predominant in wastewater sludge. In contrast, we did not observe any common structures among eight other composts produced, using the hyper-thermal composts as the inoculums, under thermophilic conditions from different materials. Principle coordinate analysis of the hyper-thermal compost indicated a large difference in bacterial community structures from material sludge and other composts. These results suggested that a distinct bacterial community structure was formed by hyper-thermal composting.

元の言語英語
ページ(範囲)606-612
ページ数7
ジャーナルJournal of Bioscience and Bioengineering
122
発行部数5
DOI
出版物ステータス出版済み - 11 1 2016

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Bacterial Structures
Composting
Soil
Hot Temperature
Sewage
Bacteroidetes
Proteobacteria
Actinobacteria
Wastewater
Waste Water
Staphylococcus
Corynebacterium
Genes
Throughput
rRNA Genes
Soils
Japan

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

これを引用

Unique hyper-thermal composting process in Kagoshima City forms distinct bacterial community structures. / Tashiro, Yukihiro; Tabata, Hanae; Itahara, Asuka; Shimizu, Natsuki; Tashiro, Kosuke; Sakai, Kenji.

:: Journal of Bioscience and Bioengineering, 巻 122, 番号 5, 01.11.2016, p. 606-612.

研究成果: ジャーナルへの寄稿記事

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abstract = "A unique compost, Satsuma soil, is produced from three types of wastewater sludge using hyper-thermal processes at temperatures much higher than that of general thermophilic processes in Kagoshima City, Japan. We analyzed the bacterial community structures of this hyper-thermal compost sample and other sludges and composts by a high-throughput barcoded pyrosequencing method targeting the 16S rRNA gene. In total, 621,076 reads were derived from 17 samples and filtered. Artificial sequences were deleted and the reads were clustered based on the operational taxonomic units (OTUs) at 97{\%} similarity. Phylum-level analysis of the hyper-thermal compost revealed drastic changes of the sludge structures (each relative abundance) from Firmicutes (average 47.8{\%}), Proteobacteria (average 22.3{\%}), and Bacteroidetes (average 10.1{\%}) to two main phyla including Firmicutes (73.6{\%}) and Actinobacteria (25.0{\%}) with less Proteobacteria (∼0.3{\%}) and Bacteroidetes (∼0.1{\%}). Furthermore, we determined the predominant species (each relative abundance) of the hyper-thermal compost including Firmicutes related to Staphylococcus cohnii (13.8{\%}), Jeotgalicoccus coquinae (8.01{\%}), and Staphylococcus lentus (5.96{\%}), and Actinobacteria related to Corynebacterium stationis (6.41{\%}), and found that these species were not predominant in wastewater sludge. In contrast, we did not observe any common structures among eight other composts produced, using the hyper-thermal composts as the inoculums, under thermophilic conditions from different materials. Principle coordinate analysis of the hyper-thermal compost indicated a large difference in bacterial community structures from material sludge and other composts. These results suggested that a distinct bacterial community structure was formed by hyper-thermal composting.",
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