Unusual feature of the T-CELL receptor genes in T-lineage acute lymphoblastic leukemia

Katsuo Yamanaka, Kazuaki Kubo, Hitoshi Kiyoi, Takuji Ichihashi, Koji Kato, Keizo Horibe, Tomoki Naoe

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

We characterized the T-cell receptor (TCR) gene rearrangements and sequences in 15 T-lineage acute lymphoblastic leukemia (T-ALL) and seven adult T-cell leukemia (ATL) samples. Southern blot analysis showed that neither of the two TCR δ alleles was deleted in two T-ALL samples, suggesting that the TCR α loci have a germ line configuration. The TCR α and β sequences were cloned and sequenced by reverse transcriptase-inverse polymerase chain reaction. Two T-ALL samples had a long complementarity determining region (CDR), three of the α chain and the other two T-ALL samples had long CDR3 of the β chain, compared with normal peripheral blood lymphocytes (PBL). Thus, a total of six T-ALL samples had unusual TCR gene structure, which was unrelated to the immunophenotype. On the other hand, CDR3 length in ATL samples was similar to normal PBL. These data suggest that T-ALL is derived from an immature T-cell repertoire which undergoes TCR gene rearrangement or has not been negatively selected.

本文言語英語
ページ(範囲)667-674
ページ数8
ジャーナルLeukemia Research
21
7
DOI
出版ステータス出版済み - 7 1 1997
外部発表はい

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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