TY - JOUR
T1 - Use of c-fos functional mapping to identify the central baroreceptor reflex pathway
T2 - Advantages and limitations
AU - Dampney, R. A.L.
AU - Li, Y. W.
AU - Hirooka, Y.
AU - Potts, P.
AU - Polson, J. W.
N1 - Funding Information:
The work of our laboratory is supported by the National Health and Medical Research Foundation of Australia, the National Heart Foundation of Australia and the Clive and Vera Ramaciotti Foundations.
Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 1995
Y1 - 1995
N2 - Prolonged stimulation of many neurons results in the expression of the immediate early gene c-fos which in turn cause the production of the protein Fos, whose presence in a cell can be detected by immunocytochemistry. This method has been used in both conscious and anaesthetized animals to identify central neurons involved in the baroreceptor reflex. In this paper we review the factors that can influence c-fos expression, with particular emphasis on the effects of different anaesthetic agents. We conclude that the c-fos method of functional mapping, when applied carefully and critically, is a very useful method of identifying central neurons that are activated by cardiovascular stimuli in conscious animals. Anaesthetic agents can significantly alter c-fos expression, and this effect differs greatly according to the type of anaesthetic used.
AB - Prolonged stimulation of many neurons results in the expression of the immediate early gene c-fos which in turn cause the production of the protein Fos, whose presence in a cell can be detected by immunocytochemistry. This method has been used in both conscious and anaesthetized animals to identify central neurons involved in the baroreceptor reflex. In this paper we review the factors that can influence c-fos expression, with particular emphasis on the effects of different anaesthetic agents. We conclude that the c-fos method of functional mapping, when applied carefully and critically, is a very useful method of identifying central neurons that are activated by cardiovascular stimuli in conscious animals. Anaesthetic agents can significantly alter c-fos expression, and this effect differs greatly according to the type of anaesthetic used.
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U2 - 10.3109/10641969509087065
DO - 10.3109/10641969509087065
M3 - Article
C2 - 7735269
AN - SCOPUS:0028871266
SN - 1064-1963
VL - 17
SP - 197
EP - 208
JO - Clinical and Experimental Hypertension
JF - Clinical and Experimental Hypertension
IS - 1-2
ER -