Use of N-methyl-N-nitrosourea treatment of fertilized egg cells for saturation mutagenesis of rice

Hikaru Satoh, Hiroaki Matsusaka, Toshihiro Kumamaru

研究成果: ジャーナルへの寄稿評論記事

23 引用 (Scopus)

抄録

The efficiency of N-methyl-N-nitrosourea (MNU) treatment of fertilized egg cells at the single-cell stage in rice (Oryza sativa L.) was about twice that of dry seeds. The proportion of recessive iso-chlorophyll mutants in the segregating progeny of M1 plants decreased remarkably with treatment at later stages of embryonic development, perhaps because of the presence of diplontic selection and chimera formation when older, multi-cellular embryos were mutagenized. Panicle sterility of the M1 plants showed a linear relationship with the frequency of chlorophyll mutations in the progeny. The mutagenicity of N-ethyl-N-nitrosourea was similar to that of MNU, but N-methyl-N′-nitro-N-nitrosoguanidine and N-ethyl-N′-nitro-N-nitrosoguanidine were less effective. Several thousand rice mutants affecting endosperm, culm length, heading date, and chlorophyll were obtained by MNU mutagenesis. The frequency of certain types of endosperm mutants seemed to differ according to the precise timing of treatment. The mutation rate was calculated as 7.4 × 10-6 per nucleotide, and the mutations were evenly distributed over the gene regions examined. These results indicate that our rice mutant library, generated by MNU mutagenesis, is a promising resource for identifying mutations in any rice gene.

元の言語英語
ページ(範囲)475-485
ページ数11
ジャーナルBreeding Science
60
発行部数5
DOI
出版物ステータス出版済み - 12 1 2010

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N-methyl-N-nitrosourea
Methylnitrosourea
Zygote
ova
mutagenesis
Mutagenesis
mutation
rice
Chlorophyll
mutants
Endosperm
chlorophyll
endosperm
Mutation Rate
N-ethyl-N-nitrosourea
mutagenicity
chimerism
Ethylnitrosourea
Methylnitronitrosoguanidine
heading

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

これを引用

Use of N-methyl-N-nitrosourea treatment of fertilized egg cells for saturation mutagenesis of rice. / Satoh, Hikaru; Matsusaka, Hiroaki; Kumamaru, Toshihiro.

:: Breeding Science, 巻 60, 番号 5, 01.12.2010, p. 475-485.

研究成果: ジャーナルへの寄稿評論記事

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