Usefulness of the ncounter analysis system to monitor immune-related biomarkers in pbmcs during anti-PD-1 Therapy

Takafumi Morisaki, Makoto Kubo, Masayo Umebayashi, Yuan Yuan, Akihiro Tsuyada, Hiroto Tanaka, Norihiro Koya, Shinichirou Nakagawa, Takashi Morisaki, Masafumi Nakamura

研究成果: ジャーナルへの寄稿学術誌査読

1 被引用数 (Scopus)

抄録

Background/Aim: Immune checkpoint inhibitors (ICIs) have dramatically changed the clinical outcomes of advanced tumours. However, biomarkers for monitoring immunological features during immunotherapy remain unclear, especially those in the peripheral blood, which are easily available. This study evaluated the usefulness of nCounter Analysis System in identifying immunological biomarkers in peripheral blood mononuclear cells (PBMCs) during ICI therapy. Patients and Methods: PBMCs from two patients who responded well to ICI therapy were used, and the expression levels of immune-related mRNA and extracellular proteins were analyzed. Results: Changes in the expression levels of 55 genes from pre-treatment to on-treatment were bioinformatically similar between the two cases. The expression levels of PD-1 were consistent with those by flow cytometry analysis, a reliable tool for monitoring various markers. Conclusion: The nCounter Analysis System may be a potent tool to simultaneously investigate genes and proteins on PBMCs as biomarkers during immunotherapy using a small amount of sample.

本文言語英語
ページ(範囲)4517-4523
ページ数7
ジャーナルAnticancer research
39
8
DOI
出版ステータス出版済み - 2019

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 癌研究

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