Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants

M. O. Abiodun, Ken Matsuoka

研究成果: 著書/レポートタイプへの貢献

抄録

Several methodologies have been employed to understand the kinetics of induced autophagic degradation in plants, but most of them are not capable of distinguishing the autophagic cargo proteins before and after induction of autophagy in cells. Here, we designed a mass photoconverter that allowed us to simultaneously monitor protein synthesis and degradation in tobacco BY-2 cells using a photoconvertible fluorescence marker protein, Kikume Green Red (KikGR). An example of a new protocol for the analysis of autophagy progression using a fusion protein of cytochrome b5 and KikGR under phosphate starvation is described. The other example described is the analysis of the proliferation of Golgi apparatus in tobacco BY-2 cells using the fusion protein of a prolyl 4-hydroxylase NtP4H1.1 and monomeric KikGR. A detailed protocol on key analysis, as well as tips and notes for experiments using KikGR proteins, are described.

元の言語英語
ホスト出版物のタイトルMethods in Enzymology
出版者Academic Press Inc.
ページ515-526
ページ数12
588
DOI
出版物ステータス出版済み - 2017

出版物シリーズ

名前Methods in Enzymology
588
ISSN(印刷物)0076-6879
ISSN(電子版)1557-7988

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Autophagy
Proteins
Tobacco
Prolyl Hydroxylases
Fusion reactions
Cytochromes b5
Cell Fusion
Golgi Apparatus
Degradation
Starvation
Proteolysis
Fluorescence
Phosphates
Kinetics
Experiments

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

これを引用

Abiodun, M. O., & Matsuoka, K. (2017). Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants. : Methods in Enzymology (巻 588, pp. 515-526). (Methods in Enzymology; 巻数 588). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2016.10.040

Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants. / Abiodun, M. O.; Matsuoka, Ken.

Methods in Enzymology. 巻 588 Academic Press Inc., 2017. p. 515-526 (Methods in Enzymology; 巻 588).

研究成果: 著書/レポートタイプへの貢献

Abiodun, MO & Matsuoka, K 2017, Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants. : Methods in Enzymology. 巻. 588, Methods in Enzymology, 巻. 588, Academic Press Inc., pp. 515-526. https://doi.org/10.1016/bs.mie.2016.10.040
Abiodun MO, Matsuoka K. Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants. : Methods in Enzymology. 巻 588. Academic Press Inc. 2017. p. 515-526. (Methods in Enzymology). https://doi.org/10.1016/bs.mie.2016.10.040
Abiodun, M. O. ; Matsuoka, Ken. / Using Photoconvertible and Extractable Fluorescent Proteins to Study Autophagy in Plants. Methods in Enzymology. 巻 588 Academic Press Inc., 2017. pp. 515-526 (Methods in Enzymology).
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