Using segmental isotope analysis of teleost fish vertebrae to estimate trophic discrimination factors of bone collagen

Jun Matsubayashi, Yu Umezawa, Michiya Matsuyama, Ryo Kawabe, Weiping Mei, Xin Wan, Atsushi Shimomae, Ichiro Tayasu

研究成果: ジャーナルへの寄稿記事

抄録

We conducted feeding experiments with sardine (Sardinops melanostictus) and Japanese flounder (Paralichthys olivaceus) to investigate trophic discrimination factors (TDFs) in bone collagen using retrospective isotope analysis. Sardines and Japanese flounder were fed a single diet for 9 months and 4 months, respectively. At the end of the experiments, we extracted vertebral centra from six sardines and seven Japanese flounder and subdivided them into multiple sections. We extracted bone collagen from each section, and measured the δ 13 C and δ 15 N of each vertebral section and food source. In all fish samples, the δ 13 C and δ 15 N values of bone collagen increased from the centers of vertebrae to the marginal sections. This trend was consistent with the predicted isotopic shift in the diets of these fishes. The TDFs for sardines were estimated at 5.58 ± 0.25‰ (mean ± SD) for δ 13 C and −0.70 ± 0.25‰ for δ 15 N; those for Japanese flounder were 3.89 ± 0.14‰ for δ 13 C and 1.18 ± 0.35‰ for δ 15 N. Our results show that segmental analysis of vertebrae can reconstruct δ 13 C and δ 15 N values for multiple life-stages of both fish species and is useful for estimating TDFs and turnover times of fish bone collagen.

元の言語英語
ページ(範囲)87-96
ページ数10
ジャーナルLimnology and Oceanography: Methods
17
発行部数2
DOI
出版物ステータス出版済み - 2 1 2019

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Collagen
Fish
Isotopes
Bone
Nutrition
Experiments

All Science Journal Classification (ASJC) codes

  • Ocean Engineering

これを引用

Using segmental isotope analysis of teleost fish vertebrae to estimate trophic discrimination factors of bone collagen. / Matsubayashi, Jun; Umezawa, Yu; Matsuyama, Michiya; Kawabe, Ryo; Mei, Weiping; Wan, Xin; Shimomae, Atsushi; Tayasu, Ichiro.

:: Limnology and Oceanography: Methods, 巻 17, 番号 2, 01.02.2019, p. 87-96.

研究成果: ジャーナルへの寄稿記事

Matsubayashi, Jun ; Umezawa, Yu ; Matsuyama, Michiya ; Kawabe, Ryo ; Mei, Weiping ; Wan, Xin ; Shimomae, Atsushi ; Tayasu, Ichiro. / Using segmental isotope analysis of teleost fish vertebrae to estimate trophic discrimination factors of bone collagen. :: Limnology and Oceanography: Methods. 2019 ; 巻 17, 番号 2. pp. 87-96.
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abstract = "We conducted feeding experiments with sardine (Sardinops melanostictus) and Japanese flounder (Paralichthys olivaceus) to investigate trophic discrimination factors (TDFs) in bone collagen using retrospective isotope analysis. Sardines and Japanese flounder were fed a single diet for 9 months and 4 months, respectively. At the end of the experiments, we extracted vertebral centra from six sardines and seven Japanese flounder and subdivided them into multiple sections. We extracted bone collagen from each section, and measured the δ 13 C and δ 15 N of each vertebral section and food source. In all fish samples, the δ 13 C and δ 15 N values of bone collagen increased from the centers of vertebrae to the marginal sections. This trend was consistent with the predicted isotopic shift in the diets of these fishes. The TDFs for sardines were estimated at 5.58 ± 0.25‰ (mean ± SD) for δ 13 C and −0.70 ± 0.25‰ for δ 15 N; those for Japanese flounder were 3.89 ± 0.14‰ for δ 13 C and 1.18 ± 0.35‰ for δ 15 N. Our results show that segmental analysis of vertebrae can reconstruct δ 13 C and δ 15 N values for multiple life-stages of both fish species and is useful for estimating TDFs and turnover times of fish bone collagen.",
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