Utilization of a PNA-peptide conjugate to induce a cancer protease-responsive RNAi effect

Eun Kyung Lee, Chan Woo Kim, Hiroyuki Kawanami, Akihiro Kishimura, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Small interfering RNA (siRNA) is regarded as a promising tool for cancer therapy because of the wide applicability to various cancer-related genes. However, non-specific delivery of siRNA is one of the major causes of adverse effects. To access the issue, here we designed a new siRNA system which turns on RNAi responding to a cancer cell-specific protease, cathepsin B. The system uses a peptide nucleic acid (PNA)-peptide conjugate to provide this protease-responsive activation. The PNA-peptides were found to form hybrids with double-stranded RNAs with complementary protruding regions, which then affected the susceptibility of dsRNA to Dicer. The dsRNA/PNA-peptide hybrids were activated in cancer cells with a high cathepsin B activity to show RNAi.

本文言語英語
ページ(範囲)85816-85821
ページ数6
ジャーナルRSC Advances
5
104
DOI
出版ステータス出版済み - 2015

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 化学工学(全般)

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