Utilization of a PNA-peptide conjugate to induce a cancer protease-responsive RNAi effect

Eun Kyung Lee, Chan Woo Kim, Hiroyuki Kawanami, Akihiro Kishimura, Takuro Niidome, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Small interfering RNA (siRNA) is regarded as a promising tool for cancer therapy because of the wide applicability to various cancer-related genes. However, non-specific delivery of siRNA is one of the major causes of adverse effects. To access the issue, here we designed a new siRNA system which turns on RNAi responding to a cancer cell-specific protease, cathepsin B. The system uses a peptide nucleic acid (PNA)-peptide conjugate to provide this protease-responsive activation. The PNA-peptides were found to form hybrids with double-stranded RNAs with complementary protruding regions, which then affected the susceptibility of dsRNA to Dicer. The dsRNA/PNA-peptide hybrids were activated in cancer cells with a high cathepsin B activity to show RNAi.

元の言語英語
ページ(範囲)85816-85821
ページ数6
ジャーナルRSC Advances
5
発行部数104
DOI
出版物ステータス出版済み - 1 1 2015

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Peptide Nucleic Acids
RNA
Peptides
Small Interfering RNA
Cathepsin B
Peptide Hydrolases
Cells
Double-Stranded RNA
Genes
Chemical activation
Cathepsins

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

これを引用

Utilization of a PNA-peptide conjugate to induce a cancer protease-responsive RNAi effect. / Lee, Eun Kyung; Kim, Chan Woo; Kawanami, Hiroyuki; Kishimura, Akihiro; Niidome, Takuro; Mori, Takeshi; Katayama, Yoshiki.

:: RSC Advances, 巻 5, 番号 104, 01.01.2015, p. 85816-85821.

研究成果: ジャーナルへの寄稿記事

Lee, Eun Kyung ; Kim, Chan Woo ; Kawanami, Hiroyuki ; Kishimura, Akihiro ; Niidome, Takuro ; Mori, Takeshi ; Katayama, Yoshiki. / Utilization of a PNA-peptide conjugate to induce a cancer protease-responsive RNAi effect. :: RSC Advances. 2015 ; 巻 5, 番号 104. pp. 85816-85821.
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