Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed

Toshihiro Kumamaru, Yuji Uemura, Yoshimi Inoue, Yoko Takemoto, Sadar Uddin Siddiqui, Masahiro Ogawa, Ikuko Hara-Nishimura, Hikaru Satoh

研究成果: ジャーナルへの寄稿記事

46 引用 (Scopus)

抄録

To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that α-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.

元の言語英語
ページ(範囲)38-46
ページ数9
ジャーナルPlant and Cell Physiology
51
発行部数1
DOI
出版物ステータス出版済み - 1 1 2010

Fingerprint

glutelins
Glutens
storage proteins
crystal structure
Seeds
Vacuoles
vacuoles
rice
enzymes
seeds
Proteins
Seedlings
seedlings
mutants
stop codon
amino acid substitution
vacuolar processing enzyme
Oryza
Terminator Codon
globulins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

これを引用

Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed. / Kumamaru, Toshihiro; Uemura, Yuji; Inoue, Yoshimi; Takemoto, Yoko; Siddiqui, Sadar Uddin; Ogawa, Masahiro; Hara-Nishimura, Ikuko; Satoh, Hikaru.

:: Plant and Cell Physiology, 巻 51, 番号 1, 01.01.2010, p. 38-46.

研究成果: ジャーナルへの寄稿記事

Kumamaru, T, Uemura, Y, Inoue, Y, Takemoto, Y, Siddiqui, SU, Ogawa, M, Hara-Nishimura, I & Satoh, H 2010, 'Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed', Plant and Cell Physiology, 巻. 51, 番号 1, pp. 38-46. https://doi.org/10.1093/pcp/pcp165
Kumamaru, Toshihiro ; Uemura, Yuji ; Inoue, Yoshimi ; Takemoto, Yoko ; Siddiqui, Sadar Uddin ; Ogawa, Masahiro ; Hara-Nishimura, Ikuko ; Satoh, Hikaru. / Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed. :: Plant and Cell Physiology. 2010 ; 巻 51, 番号 1. pp. 38-46.
@article{c4192becb4bd42e4999c54a9cb1a9ca9,
title = "Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed",
abstract = "To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that α-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.",
author = "Toshihiro Kumamaru and Yuji Uemura and Yoshimi Inoue and Yoko Takemoto and Siddiqui, {Sadar Uddin} and Masahiro Ogawa and Ikuko Hara-Nishimura and Hikaru Satoh",
year = "2010",
month = "1",
day = "1",
doi = "10.1093/pcp/pcp165",
language = "English",
volume = "51",
pages = "38--46",
journal = "Plant and Cell Physiology",
issn = "0032-0781",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed

AU - Kumamaru, Toshihiro

AU - Uemura, Yuji

AU - Inoue, Yoshimi

AU - Takemoto, Yoko

AU - Siddiqui, Sadar Uddin

AU - Ogawa, Masahiro

AU - Hara-Nishimura, Ikuko

AU - Satoh, Hikaru

PY - 2010/1/1

Y1 - 2010/1/1

N2 - To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that α-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.

AB - To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that α-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.

UR - http://www.scopus.com/inward/record.url?scp=74549197626&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=74549197626&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcp165

DO - 10.1093/pcp/pcp165

M3 - Article

VL - 51

SP - 38

EP - 46

JO - Plant and Cell Physiology

JF - Plant and Cell Physiology

SN - 0032-0781

IS - 1

ER -