Vacuolar processing enzyme plays an essential role in the crystalline structure of glutelin in rice seed

Toshihiro Kumamaru, Yuji Uemura, Yoshimi Inoue, Yoko Takemoto, Sadar Uddin Siddiqui, Masahiro Ogawa, Ikuko Hara-Nishimura, Hikaru Satoh

研究成果: Contribution to journalArticle査読

54 被引用数 (Scopus)

抄録

To identify the function of genes that regulate the processing of proglutelin, we performed an analysis of glup3 mutants, which accumulates excess amounts of proglutelin and lack the vacuolar processing enzyme (VPE). VPE activity in developing seeds from glup3 lines was reduced remarkably compared with the wild type. DNA sequencing of the VPE gene in glup3 mutants revealed either amino acid substitutions or the appearance of a stop codon within the coding region. Microscopic observations showed that α-globulin and proglutelin were distributed homogeneously within glup3 protein storage vacuoles (PSVs), and that glup3 PSVs lacked the crystalline lattice structure typical of wild-type PSVs. This suggests that the processing of proglutelin by VPE in rice is essential for proper PSV structure and compartmentalization of storage proteins. Growth retardation in glup3 seedlings was also observed, indicating that the processing of proglutelin influences early seedling development. These findings indicate that storage of glutelin in its mature form as a crystalline structure in PSVs is required for the rapid use of glutelin as a source of amino acids during early seedling development. In conclusion, VPE plays an important role in the formation of protein crystalline structures in PSVs.

本文言語英語
ページ(範囲)38-46
ページ数9
ジャーナルPlant and Cell Physiology
51
1
DOI
出版ステータス出版済み - 1 2010

All Science Journal Classification (ASJC) codes

  • 生理学
  • 植物科学
  • 細胞生物学

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