TY - JOUR
T1 - Validation of Eupatorium triplinerve Vahl Leaves, a Skin Care Herb from East Kalimantan, Using a Melanin Biosynthesis Assay
AU - Arung, Enos Tangke
AU - Kuspradini, Harlinda
AU - Kusuma, Irawan Wijaya
AU - Shimizu, Kuniyoshi
AU - Kondo, Ryuichiro
N1 - Funding Information:
This research supported was supported by the Directorate General of Higher Education, Indonesia Ministry of National Education (grant no: 436/SP2H/PP/DP2 M/VI/2010 ).
PY - 2012/4
Y1 - 2012/4
N2 - In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360μM (L-tyrosine was used as the substrate) and above 2840μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
AB - In searching for a new material made from natural resources that could be used as a whitening agent, we focused on the plants used for skin treatment by the native people of East Kalimantan. The methanol extract of the leaves of Eupatorium triplinerve Vahl showed antimelanogenesis activity in a melanin biosynthesis assay. By activity-guided fractionation, 7-methoxycoumarin (1) was isolated as an active compound. The IC50 of 1 on mushroom tyrosinase was 2360μM (L-tyrosine was used as the substrate) and above 2840μM (L-DOPA was used as the substrate), respectively. Regarding melanin formation inhibition in B16 melanoma cells, the IC50 of 1 was 1780μM with 83% cell viability at IC50. Based on these results, we validated that the leaf extract is in line with the traditional use of the Dayak tribe in East Kalimantan.
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U2 - 10.1016/j.jams.2012.01.003
DO - 10.1016/j.jams.2012.01.003
M3 - Article
C2 - 22483187
AN - SCOPUS:84859420097
VL - 5
SP - 87
EP - 92
JO - JAMS Journal of Acupuncture and Meridian Studies
JF - JAMS Journal of Acupuncture and Meridian Studies
SN - 2005-2901
IS - 2
ER -