Variants of C-C motif chemokine 22 (CCL22) are associated with susceptibility to atopic dermatitis: Case-control studies

Tomomitsu Hirota, Hidehisa Saeki, Kaori Tomita, Shota Tanaka, Kouji Ebe, Masafumi Sakashita, Takechiyo Yamada, Shigeharu Fujieda, Akihiko Miyatake, Satoru Doi, Tadao Enomoto, Nobuyuki Hizawa, Tohru Sakamoto, Hironori Masuko, Takashi Sasaki, Tamotsu Ebihara, Masayuki Amagai, Hitokazu Esaki, Satoshi Takeuchi, Masutaka FurueEmiko Noguchi, Naoyuki Kamatani, Yusuke Nakamura, Michiaki Kubo, Mayumi Tamari

研究成果: Contribution to journalArticle査読

13 被引用数 (Scopus)

抄録

Atopic dermatitis (AD) is a common inflammatory skin disease caused by multiple genetic and environmental factors. AD is characterized by the local infiltration of T helper type 2 (Th2) cells. Recent clinical studies have shown important roles of the Th2 chemokines, CCL22 and CCL17 in the pathogenesis of AD. To investigate whether polymorphisms of the CCL22 gene affect the susceptibility to AD, we conducted association studies and functional studies of the related variants. We first resequenced the CCL22 gene and found a total of 39 SNPs. We selected seven tag SNPs in the CCL22 gene, and conducted association studies using two independent Japanese populations (1 st population, 916 cases and 1,032 controls; 2 nd population 1,034 cases and 1,004 controls). After the association results were combined by inverse variance method, we observed a significant association at rs4359426 (meta-analysis, combined P = 9.6×10 -6; OR, 0.74; 95% CI, 0.65-0.85). Functional analysis revealed that the risk allele of rs4359426 contributed to higher expression levels of CCL22 mRNA. We further examined the allelic differences in the binding of nuclear proteins by electrophoretic mobility shift assay. The signal intensity of the DNA-protein complex derived from the G allele of rs223821, which was in absolute LD with rs4359426, was higher than that from the A allele. Although further functional analyses are needed, it is likely that related variants play a role in susceptibility to AD in a gain-of-function manner. Our findings provide a new insight into the etiology and pathogenesis of AD.

本文言語英語
論文番号e26987
ジャーナルPloS one
6
11
DOI
出版ステータス出版済み - 11 17 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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