Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity

Masayuki Sugawara, Satoko Takahashi, Yosuke Umehara, Hiroya Iwano, Hirohito Tsurumaru, Haruka Odake, Yuta Suzuki, Hitoshi Kondo, Yuki Konno, Takeo Yamakawa, Shusei Sato, Hisayuki Mitsui, Kiwamu Minamisawa

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

Genotype-specific incompatibility in legume–rhizobium symbiosis has been suggested to be controlled by effector-triggered immunity underlying pathogenic host-bacteria interactions. However, the rhizobial determinant interacting with the host resistance protein (e.g., Rj2) and the molecular mechanism of symbiotic incompatibility remain unclear. Using natural mutants of Bradyrhizobium diazoefficiens USDA 122, we identified a type III-secretory protein NopP as the determinant of symbiotic incompatibility with Rj2-soybean. The analysis of nopP mutations and variants in a culture collection reveal that three amino acid residues (R60, R67, and H173) in NopP are required for Rj2-mediated incompatibility. Complementation of rj2-soybean by the Rj2 allele confers the incompatibility induced by USDA 122-type NopP. In response to incompatible strains, Rj2-soybean plants activate defense marker gene PR-2 and suppress infection thread number at 2 days after inoculation. These results suggest that Rj2-soybeans monitor the specific variants of NopP and reject bradyrhizobial infection via effector-triggered immunity mediated by Rj2 protein.

元の言語英語
記事番号3139
ジャーナルNature communications
9
発行部数1
DOI
出版物ステータス出版済み - 12 1 2018

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effectors
soybeans
incompatibility
immunity
Soybeans
Immunity
United States Department of Agriculture
infectious diseases
Proteins
proteins
determinants
Bradyrhizobium
symbiosis
Symbiosis
Bacteria
Genes
Infection
threads
Amino Acids
inoculation

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

これを引用

Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity. / Sugawara, Masayuki; Takahashi, Satoko; Umehara, Yosuke; Iwano, Hiroya; Tsurumaru, Hirohito; Odake, Haruka; Suzuki, Yuta; Kondo, Hitoshi; Konno, Yuki; Yamakawa, Takeo; Sato, Shusei; Mitsui, Hisayuki; Minamisawa, Kiwamu.

:: Nature communications, 巻 9, 番号 1, 3139, 01.12.2018.

研究成果: ジャーナルへの寄稿記事

Sugawara, M, Takahashi, S, Umehara, Y, Iwano, H, Tsurumaru, H, Odake, H, Suzuki, Y, Kondo, H, Konno, Y, Yamakawa, T, Sato, S, Mitsui, H & Minamisawa, K 2018, 'Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity', Nature communications, 巻. 9, 番号 1, 3139. https://doi.org/10.1038/s41467-018-05663-x
Sugawara, Masayuki ; Takahashi, Satoko ; Umehara, Yosuke ; Iwano, Hiroya ; Tsurumaru, Hirohito ; Odake, Haruka ; Suzuki, Yuta ; Kondo, Hitoshi ; Konno, Yuki ; Yamakawa, Takeo ; Sato, Shusei ; Mitsui, Hisayuki ; Minamisawa, Kiwamu. / Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity. :: Nature communications. 2018 ; 巻 9, 番号 1.
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abstract = "Genotype-specific incompatibility in legume–rhizobium symbiosis has been suggested to be controlled by effector-triggered immunity underlying pathogenic host-bacteria interactions. However, the rhizobial determinant interacting with the host resistance protein (e.g., Rj2) and the molecular mechanism of symbiotic incompatibility remain unclear. Using natural mutants of Bradyrhizobium diazoefficiens USDA 122, we identified a type III-secretory protein NopP as the determinant of symbiotic incompatibility with Rj2-soybean. The analysis of nopP mutations and variants in a culture collection reveal that three amino acid residues (R60, R67, and H173) in NopP are required for Rj2-mediated incompatibility. Complementation of rj2-soybean by the Rj2 allele confers the incompatibility induced by USDA 122-type NopP. In response to incompatible strains, Rj2-soybean plants activate defense marker gene PR-2 and suppress infection thread number at 2 days after inoculation. These results suggest that Rj2-soybeans monitor the specific variants of NopP and reject bradyrhizobial infection via effector-triggered immunity mediated by Rj2 protein.",
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