抄録
Induced pluripotent stem cells (iPSCs) are a promising tool for regenerative medicine. Use of iPSC lines for future hematotherapy will require examination of their hematopoietic potential. Adult skin fibroblast somatic cells constitute a source of iPSCs that can be accessed clinically without ethical issues. Here, we used different methods to compare mesodermal and hematopoietic potential by embryoid body formation of five iPSC lines established from adult mouse tail-tip fibroblasts (TTFs). We observed variation in proliferation and in expression of genes (Brachyury, Tbx1, Gata1, Klf1, Csf1r) and proteins (Flk1, Ter119 and CD45) among TTF-derived lines. 256H18 iPSCs showed highest proliferation and most efficient differentiation into mesodermal and hematopoietic cells, while expression levels of the pluripotency genes Oct3/4, Sox2, Klf4 and Nanog were lowest among lines analyzed. By contrast, the 212B2 line, transduced with c-Myc, showed lowest proliferation and differentiation potential, although expression levels of Oct3/4, Sox2 and Klf4 were highest. Overall, we find that mesodermal and hematopoietic potential varies among iPSCs from an identical tissue source and that c-Myc expression likely underlies these differences.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 958-968 |
| ページ数 | 11 |
| ジャーナル | Stem Cell Reviews and Reports |
| 巻 | 7 |
| 発行部数 | 4 |
| DOI | |
| 出版物ステータス | 出版済み - 11 1 2011 |
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All Science Journal Classification (ASJC) codes
- Cell Biology
- Cancer Research
これを引用
Variation in Mesodermal and Hematopoietic Potential of Adult Skin-derived Induced Pluripotent Stem Cell Lines in Mice. / Inoue, Tomoko; Kulkeaw, Kasem; Okayama, Satoko; Tani, Kenzaburo; Sugiyama, Daisuke.
：: Stem Cell Reviews and Reports, 巻 7, 番号 4, 01.11.2011, p. 958-968.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Variation in Mesodermal and Hematopoietic Potential of Adult Skin-derived Induced Pluripotent Stem Cell Lines in Mice
AU - Inoue, Tomoko
AU - Kulkeaw, Kasem
AU - Okayama, Satoko
AU - Tani, Kenzaburo
AU - Sugiyama, Daisuke
PY - 2011/11/1
Y1 - 2011/11/1
N2 - Induced pluripotent stem cells (iPSCs) are a promising tool for regenerative medicine. Use of iPSC lines for future hematotherapy will require examination of their hematopoietic potential. Adult skin fibroblast somatic cells constitute a source of iPSCs that can be accessed clinically without ethical issues. Here, we used different methods to compare mesodermal and hematopoietic potential by embryoid body formation of five iPSC lines established from adult mouse tail-tip fibroblasts (TTFs). We observed variation in proliferation and in expression of genes (Brachyury, Tbx1, Gata1, Klf1, Csf1r) and proteins (Flk1, Ter119 and CD45) among TTF-derived lines. 256H18 iPSCs showed highest proliferation and most efficient differentiation into mesodermal and hematopoietic cells, while expression levels of the pluripotency genes Oct3/4, Sox2, Klf4 and Nanog were lowest among lines analyzed. By contrast, the 212B2 line, transduced with c-Myc, showed lowest proliferation and differentiation potential, although expression levels of Oct3/4, Sox2 and Klf4 were highest. Overall, we find that mesodermal and hematopoietic potential varies among iPSCs from an identical tissue source and that c-Myc expression likely underlies these differences.
AB - Induced pluripotent stem cells (iPSCs) are a promising tool for regenerative medicine. Use of iPSC lines for future hematotherapy will require examination of their hematopoietic potential. Adult skin fibroblast somatic cells constitute a source of iPSCs that can be accessed clinically without ethical issues. Here, we used different methods to compare mesodermal and hematopoietic potential by embryoid body formation of five iPSC lines established from adult mouse tail-tip fibroblasts (TTFs). We observed variation in proliferation and in expression of genes (Brachyury, Tbx1, Gata1, Klf1, Csf1r) and proteins (Flk1, Ter119 and CD45) among TTF-derived lines. 256H18 iPSCs showed highest proliferation and most efficient differentiation into mesodermal and hematopoietic cells, while expression levels of the pluripotency genes Oct3/4, Sox2, Klf4 and Nanog were lowest among lines analyzed. By contrast, the 212B2 line, transduced with c-Myc, showed lowest proliferation and differentiation potential, although expression levels of Oct3/4, Sox2 and Klf4 were highest. Overall, we find that mesodermal and hematopoietic potential varies among iPSCs from an identical tissue source and that c-Myc expression likely underlies these differences.
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UR - http://www.scopus.com/inward/citedby.url?scp=82355163503&partnerID=8YFLogxK
U2 - 10.1007/s12015-011-9249-3
DO - 10.1007/s12015-011-9249-3
M3 - Article
C2 - 21424235
AN - SCOPUS:82355163503
VL - 7
SP - 958
EP - 968
JO - Stem Cell Reviews
JF - Stem Cell Reviews
SN - 1550-8943
IS - 4
ER -