Variation in the structural changes of myoglobin in the presence of several protic ionic liquid

Pankaj Attri, Indrani Jha, Eun Ha Choi, Pannuru Venkatesu

研究成果: ジャーナルへの寄稿学術誌査読

44 被引用数 (Scopus)

抄録

Protein stability in ionic solution depends on the delicate balance between protein-ion and ion-ion interactions. To address the ion specific effects on the protein, we have examined the stability of myoglobin (Mb) in the presence of buffer and ammonium-based ionic liquids (ILs) (50%, v/v). Here, fluorescence and circular dichroism (CD) spectroscopy experiments are used to study the influence of ILs on structure and stability of Mb. Our experimental results reveal that more viscous ILs (sulphate or phosphate ions) are stabilizers and therefore more biocompatible for Mb structure. Surprisingly, the less viscous ILs such as acetate anion based ILs are destabilizers for the native structure of Mb. Our results explicitly elucidate that anion variation has significant influence on Mb stability efficiency than cation variation. This study provides insight into anion effects on protein stability and explains that the intrasolvent interactions can be leveraged to enhance the stability.

本文言語英語
ページ(範囲)114-123
ページ数10
ジャーナルInternational Journal of Biological Macromolecules
69
DOI
出版ステータス出版済み - 8月 2014
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 構造生物学
  • 生化学
  • 分子生物学
  • 経済学、計量経済学
  • エネルギー(全般)

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