Vascular network formation for a long-term spheroid culture by co-culturing endothelial cells and fibroblasts

Tomoya Hayashi, Hisako Imamura, Koichi Nishiyama, Hirofumi Shintaku, Hidetoshi Kotera, Takashi Miura, Ryuji Yokokawa

研究成果: Contribution to journalConference article査読

5 被引用数 (Scopus)

抄録

In this paper, we present a poly-dimethylsiloxane (PDMS) microfluidic device to create a vascular network for a long-term spheroid culture, of which network and spheroid are consist of human umbilical vein endothelial cells (HUVEC) and normal human lung fibroblasts (LF), respectively. Following device design, fabrication, and fundamental evaluation of HUVEC sprouting conditions, we visualized that HUVEC networks were successfully formed by the co-culture with LFs and reached a LF-based spheroid. Moreover, perfusability of the network was evaluated by injecting fluorescent microbeads. This platform will be applicable for long-term tissue cultures to understand morphogenesis and modeling of blood vessel functions.

本文言語英語
論文番号7050995
ページ(範囲)476-479
ページ数4
ジャーナルProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
2015-February
February
DOI
出版ステータス出版済み - 1 1 2015
イベント2015 28th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2015 - Estoril, ポルトガル
継続期間: 1 18 20151 22 2015

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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