Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca2+ concentration in isolated rat aorta

Thanutchaporn Kumrungsee, Sayaka Akiyama, Tomomi Saiki, Masato Omae, Kazuhiro Hamasawa, Toshiro Matsui

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

Our study is the first to demonstrate the vasorelaxant effect of Candida utilis yeast extract on rat aorta (EC50 of 7.2 ± 3.2 mg/mL). Among five identified compounds, 5′-methylthioadenosine (MTA) exhibited comparable vasorelaxant effect (EC50 of 190 ± 40 μM) with adenosine, a known vasodilator, on 1 μM phenylephrine (PE)-contracted Sprague-Dawley rat aortic rings. MTA induced vasorelaxation in an endothelium-independent manner and independent of the adenosine receptors. MTA reduced a CaCl2-induced vasocontraction stimulated by 1 μM PE, whereas the effect was abolished in a 60 mM KCl-induced vasocontraction. This indicates that MTA was not involved in the suppression of extracellular Ca2+ influx. MTA significantly (P < 0.01) attenuated the PE-induced activation of calmodulin-dependent kinase II (CaMK II) in aortic rings and inhibited the phosphorylation of L-type Ca2+ channel (VDCC). In conclusion, the underlying mechanism(s) of MTA-induced vasorelaxation involves the inhibition of Ca2+/CaMK II/VDCC phosphorylation pathway, resulting in the suppression of intracellular Ca2+ concentration in aortic rings.

本文言語英語
ページ(範囲)3362-3370
ページ数9
ジャーナルJournal of Agricultural and Food Chemistry
64
17
DOI
出版ステータス出版済み - 5 4 2016

All Science Journal Classification (ASJC) codes

  • 化学 (全般)
  • 農業および生物科学(全般)

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「Vasorelaxant effect of 5′-methylthioadenosine obtained from candida utilis yeast extract through the suppression of intracellular Ca<sup>2+</sup> concentration in isolated rat aorta」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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