Ventromedial hypothalamic nucleus-specific enhancer of Ad4BP/SF-1 gene

Yuichi Shima, Mohamad Zubair, Satoru Ishihara, Yuko Shinohara, Sanae Oka, Shioko Kimura, Shiki Okamoto, Yasuhiko Minokoshi, Sachiyo Suita, Ken Ichirou Morohashi

研究成果: Contribution to journalArticle査読

35 被引用数 (Scopus)

抄録

Ad4BP/SF-1 [Ad4 binding protein/steroidogenic factor-1 (designated NR5A1)] is a transcription factor essential for animal reproduction. Based on the phenotypes observed in gene-disrupted mice, Ad4BP/SF-1 is thought to be involved in establishment of the hypothalamic-pituitary-gonadal axis. However, the mechanisms underlying tissue-specific expression of Ad4BP/SF-1 are largely unknown. Here, we investigated the cis-regulatory regions of the mouse Ad4BP/SF-1 gene by transgenic mouse assays, and identified a ventromedial hypothalamic nucleus (VMH)-specific enhancer. The enhancer localized in intron 6 is highly conserved between mouse, human, and chick. The enhancer has the potential to reproduce endogenous gene expression from the fetal ventromedial diencephalon to the adult VMH. The VMH enhancer was characterized by the presence of suppressive and activating elements. Mutation of the former element resulted in ectopic lacZ reporter gene expression in an area dorsal to the intrinsic expression domain and in the ventricular zone, whereas mutations in the latter containing ATTA motifs led to the disappearance of the reporter gene expression, suggesting the involvement of homeobox proteins. Using nuclear extracts prepared from the adult hypothalami, EMSAs identified specific protein binding to the activating elements but not to the suppressive element.

本文言語英語
ページ(範囲)2812-2823
ページ数12
ジャーナルMolecular Endocrinology
19
11
DOI
出版ステータス出版済み - 11 2005
外部発表はい

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Endocrinology

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