VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae

Non Miyata, Takuya Miyoshi, Takanori Yamaguchi, Toshimitsu Nakazono, Motohiro Tani, Osamu Kuge

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Phosphatidylethanolamine (PE) in the yeast Saccharomyces cerevisiae is synthesized through decarboxylation of phosphatidylserine (PS), catalysed by PS decarboxylase 1 (Psd1p) and 2 (Psd2p) and the cytidine 5-diphosphate (CDP)-ethanolamine (CDP-Etn) pathway. PSD1 null (psd1δ) and PSD2 null (psd2δ) mutants are viable in a synthetic minimal medium, but a psd1δ psd2δ double mutant exhibits Etn auxotrophy, which is incorporated into PE through the CDP-Etn pathway. We have previously shown that psd1δ is synthetic lethal with deletion of VID22 (vid22δ) [Kuroda et al. (2011) Mol. Microbiol. 80, 248- 265]. In the present study, we found that vid22δ mutant exhibits Etn auxotrophy under PSD1-depressed conditions. Deletion of VID22 in wild-Type and PSD1-depressed cells caused partial defects in PE formation through decarboxylation of PS. The enzyme activity of PS decarboxylase in an extract of vid22δ cells was 70% of that in wild-Type cells and similar to that in psd2δ cells and the PS decarboxylase activity remaining in the PSD1-depressed cells became almost negligible with deletion of VID22. Thus, the vid22δ mutation was suggested to cause a defect in the Psd2p activity. Furthermore, vid22δ cells were shown to be defective in expression of the PSD2 gene tagged with 6×HA, the defect being ameliorated by replacement of the native promoter of the PSD2 gene with a CYC1 promoter. In addition, an α-galactosidase reporter assay revealed that the activity of the promoter of the PSD2 gene in vid22δ cells was 5% of that in wild-Type cells. These results showed that VID22 is required for transcriptional activation of the PSD2 gene.

元の言語英語
ページ(範囲)319-328
ページ数10
ジャーナルBiochemical Journal
472
発行部数3
DOI
出版物ステータス出版済み - 12 15 2015

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Yeast
Transcriptional Activation
Saccharomyces cerevisiae
Genes
Yeasts
Chemical activation
Cytidine
Diphosphates
Phosphatidylserines
Defects
Galactosidases
Decarboxylation
Ethanolamine
Enzyme activity
Assays
Cytidine Diphosphate
Cell Extracts
phosphatidylserine decarboxylase
phosphatidylethanolamine
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae. / Miyata, Non; Miyoshi, Takuya; Yamaguchi, Takanori; Nakazono, Toshimitsu; Tani, Motohiro; Kuge, Osamu.

:: Biochemical Journal, 巻 472, 番号 3, 15.12.2015, p. 319-328.

研究成果: ジャーナルへの寄稿記事

Miyata, Non ; Miyoshi, Takuya ; Yamaguchi, Takanori ; Nakazono, Toshimitsu ; Tani, Motohiro ; Kuge, Osamu. / VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae. :: Biochemical Journal. 2015 ; 巻 472, 番号 3. pp. 319-328.
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title = "VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae",
abstract = "Phosphatidylethanolamine (PE) in the yeast Saccharomyces cerevisiae is synthesized through decarboxylation of phosphatidylserine (PS), catalysed by PS decarboxylase 1 (Psd1p) and 2 (Psd2p) and the cytidine 5-diphosphate (CDP)-ethanolamine (CDP-Etn) pathway. PSD1 null (psd1δ) and PSD2 null (psd2δ) mutants are viable in a synthetic minimal medium, but a psd1δ psd2δ double mutant exhibits Etn auxotrophy, which is incorporated into PE through the CDP-Etn pathway. We have previously shown that psd1δ is synthetic lethal with deletion of VID22 (vid22δ) [Kuroda et al. (2011) Mol. Microbiol. 80, 248- 265]. In the present study, we found that vid22δ mutant exhibits Etn auxotrophy under PSD1-depressed conditions. Deletion of VID22 in wild-Type and PSD1-depressed cells caused partial defects in PE formation through decarboxylation of PS. The enzyme activity of PS decarboxylase in an extract of vid22δ cells was 70{\%} of that in wild-Type cells and similar to that in psd2δ cells and the PS decarboxylase activity remaining in the PSD1-depressed cells became almost negligible with deletion of VID22. Thus, the vid22δ mutation was suggested to cause a defect in the Psd2p activity. Furthermore, vid22δ cells were shown to be defective in expression of the PSD2 gene tagged with 6×HA, the defect being ameliorated by replacement of the native promoter of the PSD2 gene with a CYC1 promoter. In addition, an α-galactosidase reporter assay revealed that the activity of the promoter of the PSD2 gene in vid22δ cells was 5{\%} of that in wild-Type cells. These results showed that VID22 is required for transcriptional activation of the PSD2 gene.",
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T1 - VID22 is required for transcriptional activation of the PSD2 gene in the yeast Saccharomyces cerevisiae

AU - Miyata, Non

AU - Miyoshi, Takuya

AU - Yamaguchi, Takanori

AU - Nakazono, Toshimitsu

AU - Tani, Motohiro

AU - Kuge, Osamu

PY - 2015/12/15

Y1 - 2015/12/15

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