Virulence of lipopolysaccharide-deficient mutants of serratia liquefaciens toward the silkworm, Bombyx mori

研究成果: ジャーナルへの寄稿記事

抄録

We aimed to identify virulence-associated genes of Serratia liquefaciens FK01 against Bombyx mori. Among 1,200 transconjugants from a transposon library, 4 (ET0234, ET0373, ET0418, and ET0964) showed decreased virulence towards B. mori. Southern hybridization revealed that the transposon was inserted at a single site of the S. liquefaciens genome. The flanking sequences of the transposon indicated that it disrupted the lipopolysaccharide (LPS) synthesis gene in all mutants. The complemented strain restored virulence completely or partially. Thus, LPS contributes to the virulence of S. liquefaciens against B. mori. Serum killing assays indicated that the bacterium was probably killed by the complement system. Since the innate immunity of a host is triggered by bacterial recognition, LPS might inhibit recognition by modifying the bacterial surface in B. mori.

元の言語英語
ページ(範囲)7-14
ページ数8
ジャーナルJournal of Insect Biotechnology and Sericology
85
発行部数1
DOI
出版物ステータス出版済み - 4 28 2016

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Serratia liquefaciens
Serratia
Bombyx
Bombyx mori
silkworms
lipopolysaccharides
Virulence
Lipopolysaccharides
virulence
Genes
transposons
mutants
Assays
Bacteria
Innate Immunity
Southern blotting
Libraries
complement
genes
Genome

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

これを引用

@article{ecd315e55e914fdda78a1ea8a6a3a52c,
title = "Virulence of lipopolysaccharide-deficient mutants of serratia liquefaciens toward the silkworm, Bombyx mori",
abstract = "We aimed to identify virulence-associated genes of Serratia liquefaciens FK01 against Bombyx mori. Among 1,200 transconjugants from a transposon library, 4 (ET0234, ET0373, ET0418, and ET0964) showed decreased virulence towards B. mori. Southern hybridization revealed that the transposon was inserted at a single site of the S. liquefaciens genome. The flanking sequences of the transposon indicated that it disrupted the lipopolysaccharide (LPS) synthesis gene in all mutants. The complemented strain restored virulence completely or partially. Thus, LPS contributes to the virulence of S. liquefaciens against B. mori. Serum killing assays indicated that the bacterium was probably killed by the complement system. Since the innate immunity of a host is triggered by bacterial recognition, LPS might inhibit recognition by modifying the bacterial surface in B. mori.",
author = "Erika Taira and Hiroaki Mon and Man Lee and Takahiro Kusakabe and Chisa Yasunaga-Aoki and Kazuhiro Iiyama",
year = "2016",
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journal = "Journal of Insect Biotechnology and Sericology",
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T1 - Virulence of lipopolysaccharide-deficient mutants of serratia liquefaciens toward the silkworm, Bombyx mori

AU - Taira, Erika

AU - Mon, Hiroaki

AU - Lee, Man

AU - Kusakabe, Takahiro

AU - Yasunaga-Aoki, Chisa

AU - Iiyama, Kazuhiro

PY - 2016/4/28

Y1 - 2016/4/28

N2 - We aimed to identify virulence-associated genes of Serratia liquefaciens FK01 against Bombyx mori. Among 1,200 transconjugants from a transposon library, 4 (ET0234, ET0373, ET0418, and ET0964) showed decreased virulence towards B. mori. Southern hybridization revealed that the transposon was inserted at a single site of the S. liquefaciens genome. The flanking sequences of the transposon indicated that it disrupted the lipopolysaccharide (LPS) synthesis gene in all mutants. The complemented strain restored virulence completely or partially. Thus, LPS contributes to the virulence of S. liquefaciens against B. mori. Serum killing assays indicated that the bacterium was probably killed by the complement system. Since the innate immunity of a host is triggered by bacterial recognition, LPS might inhibit recognition by modifying the bacterial surface in B. mori.

AB - We aimed to identify virulence-associated genes of Serratia liquefaciens FK01 against Bombyx mori. Among 1,200 transconjugants from a transposon library, 4 (ET0234, ET0373, ET0418, and ET0964) showed decreased virulence towards B. mori. Southern hybridization revealed that the transposon was inserted at a single site of the S. liquefaciens genome. The flanking sequences of the transposon indicated that it disrupted the lipopolysaccharide (LPS) synthesis gene in all mutants. The complemented strain restored virulence completely or partially. Thus, LPS contributes to the virulence of S. liquefaciens against B. mori. Serum killing assays indicated that the bacterium was probably killed by the complement system. Since the innate immunity of a host is triggered by bacterial recognition, LPS might inhibit recognition by modifying the bacterial surface in B. mori.

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