Virulence of lipopolysaccharide-deficient mutants of serratia liquefaciens toward the silkworm, Bombyx mori

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

We aimed to identify virulence-associated genes of Serratia liquefaciens FK01 against Bombyx mori. Among 1,200 transconjugants from a transposon library, 4 (ET0234, ET0373, ET0418, and ET0964) showed decreased virulence towards B. mori. Southern hybridization revealed that the transposon was inserted at a single site of the S. liquefaciens genome. The flanking sequences of the transposon indicated that it disrupted the lipopolysaccharide (LPS) synthesis gene in all mutants. The complemented strain restored virulence completely or partially. Thus, LPS contributes to the virulence of S. liquefaciens against B. mori. Serum killing assays indicated that the bacterium was probably killed by the complement system. Since the innate immunity of a host is triggered by bacterial recognition, LPS might inhibit recognition by modifying the bacterial surface in B. mori.

本文言語英語
ページ(範囲)7-14
ページ数8
ジャーナルJournal of Insect Biotechnology and Sericology
85
1
DOI
出版ステータス出版済み - 4 28 2016

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー
  • ビジネス、管理および会計(全般)
  • 農業および生物科学(全般)
  • 昆虫科学
  • 産業および生産工学

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