Virulence of phospholipase C mutants of Pseudomonas aeruginosa PAO1 against the silkworm, Bombyx mori

研究成果: ジャーナルへの寄稿記事

抄録

To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against Bombyx mori, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, GmR) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1 plcB, and PAO1 plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of Bombyx mori larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.

元の言語英語
ページ(範囲)115-120
ページ数6
ジャーナルJournal of Insect Biotechnology and Sericology
77
発行部数2
出版物ステータス出版済み - 6 1 2008

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Bombyx
phospholipase C
Type C Phospholipases
Bombyx mori
silkworms
Pseudomonas aeruginosa
Virulence
virulence
Genes
mutants
inactivation
Larva
Galleria mellonella
culture filtrates
Southern blotting
genes
instars
Lepidoptera
Growth
Silkworm

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Business, Management and Accounting(all)
  • Agricultural and Biological Sciences(all)
  • Insect Science
  • Industrial and Manufacturing Engineering

これを引用

@article{11290337de264a1b9738536d66da15fc,
title = "Virulence of phospholipase C mutants of Pseudomonas aeruginosa PAO1 against the silkworm, Bombyx mori",
abstract = "To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against Bombyx mori, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, GmR) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1 plcB, and PAO1 plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of Bombyx mori larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.",
author = "Kazuhiro Iiyama and Yuuka Chieda and Man Lee and Takahiro Kusakabe and Chisa Yasunaga-Aoki and Susumu Shimizu",
year = "2008",
month = "6",
day = "1",
language = "English",
volume = "77",
pages = "115--120",
journal = "Journal of Insect Biotechnology and Sericology",
issn = "1346-8073",
publisher = "Japanese Society of Sericultural Sciences",
number = "2",

}

TY - JOUR

T1 - Virulence of phospholipase C mutants of Pseudomonas aeruginosa PAO1 against the silkworm, Bombyx mori

AU - Iiyama, Kazuhiro

AU - Chieda, Yuuka

AU - Lee, Man

AU - Kusakabe, Takahiro

AU - Yasunaga-Aoki, Chisa

AU - Shimizu, Susumu

PY - 2008/6/1

Y1 - 2008/6/1

N2 - To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against Bombyx mori, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, GmR) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1 plcB, and PAO1 plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of Bombyx mori larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.

AB - To estimate the contribution of phospholipase C (PLC) in the virulence of Pseudomonas aeruginosa against Bombyx mori, PlcB, PlcH, and PlcN mutants were created by the sacB-based counterselection method. Insertion of Ωaac cassette (selection marker, GmR) in plcB, plcH, and plcN was confirmed by Southern hybridization and the mutants were designated as PAO1plcB, PAO1plcH, and PAO1plcN, respectively. Inactivation of PLC genes did not cause growth deficient in vitro. The culture filtrates of PAO1, PAO1 plcB, and PAO1 plcN had hemolytic activity, but not PAO1plcH. When the PLC mutants were inoculated into the fourth instar of Bombyx mori larvae, all mutants maintained high virulence, indicating that at least inactivation of a single plc gene in P. aeruginosa did not affect the virulence against B. mori. It was different from the results obtained in another Lepidopteran host model, Galleria mellonella.

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