金属微粒子シートによる細胞接着ナノ界面の可視化

増田 志穂美, 玉田 薫

研究成果: Contribution to journalArticle

抄録

In this paper, we report a simple, effective method for the visualization of a cell–attached nanointerface using localized surface plasmon resonance (LSPR) excited on a self–assembled metal nanoparticle sheet. The LSPR on the nanoparticle sheet provides high–contrast interfacial images due to the confined light at the nanointerface. The experiment of immobilized RBL–2H3 cells with fluorescence–labeled actin filaments revealed high axial resolution for the focal adhesion even under a regular epifluorescence microscope. This imaging technique can be applied even for live–cell imaging under a total internal reflection fluorescence (TIRF) microscope. This non–scanning–type, high–resolution imaging method will be an effective tool for monitoring interfacial phenomena including molecular level of rapid reaction or various cellular and molecular dynamics.
寄稿の翻訳タイトルVisualization of Cell Attached Nanointerface using Metal Nanoparticle Sheet
本文言語日本語
ページ(範囲)115-120
ページ数6
ジャーナルMembrane
45
3
DOI
出版ステータス出版済み - 2020

フィンガープリント

「金属微粒子シートによる細胞接着ナノ界面の可視化」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル