Visualization of intracellular lipid metabolism in brown adipocytes by time-lapse ultra-multiplex CARS microspectroscopy with an onstage incubator

Yuki Takei, Rie Hirai, Aya Fukuda, Shinichi Miyazaki, Rintaro Shimada, Yuko Okamatsu-Ogura, Masayuki Saito, Philippe Leproux, Koji Hisatake, Hideaki Kano

研究成果: Contribution to journalArticle査読

抄録

We visualized a dynamic process of fatty acid uptake of brown adipocytes using a time-lapse ultra-broadband multiplex coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging system with an onstage incubator. Combined with the deuterium labeling technique, the intracellular uptake of saturated fatty acids was traced up to 9 h, a substantial advance over the initial multiplex CARS system, with an analysis time of 80 min. Characteristic metabolic activities of brown adipocytes, such as resistance to lipid saturation, were elucidated, supporting the utility of the newly developed system.

本文言語英語
論文番号125102
ジャーナルJournal of Chemical Physics
155
12
DOI
出版ステータス出版済み - 9 28 2021

All Science Journal Classification (ASJC) codes

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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