Vitellogenin Incorporation into Oocytes of Rainbow Trout, Oncorhynchus mykiss, in Vitro: Effect of Hormones on Denuded Oocytes: vitellogenin/oocyte growth/insulin/thyroxine/rainbow trout

Naoki Shibata, Michiyasu Yoshikuni, Yoshitaka Nagahama

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13% and 12%, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.

元の言語英語
ページ(範囲)115-121
ページ数7
ジャーナルDevelopment, Growth & Differentiation
35
発行部数1
DOI
出版物ステータス出版済み - 1 1 1993

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Vitellogenins
Oncorhynchus mykiss
Thyroxine
Oocytes
Hormones
Insulin
Growth
Gonadotropins
Ovarian Follicle
In Vitro Techniques
Thyroid Hormones
Prolactin
Growth Hormone
Testosterone

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

これを引用

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title = "Vitellogenin Incorporation into Oocytes of Rainbow Trout, Oncorhynchus mykiss, in Vitro: Effect of Hormones on Denuded Oocytes: vitellogenin/oocyte growth/insulin/thyroxine/rainbow trout",
abstract = "An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13{\%} and 12{\%}, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.",
author = "Naoki Shibata and Michiyasu Yoshikuni and Yoshitaka Nagahama",
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T1 - Vitellogenin Incorporation into Oocytes of Rainbow Trout, Oncorhynchus mykiss, in Vitro

T2 - Effect of Hormones on Denuded Oocytes: vitellogenin/oocyte growth/insulin/thyroxine/rainbow trout

AU - Shibata, Naoki

AU - Yoshikuni, Michiyasu

AU - Nagahama, Yoshitaka

PY - 1993/1/1

Y1 - 1993/1/1

N2 - An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13% and 12%, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.

AB - An in vitro culture procedure to measure vitellogenin (VTG) incorporation into oocytes without follicle cell layers was developed using oocytes of the rainbow trout, Oncorhynchus mykiss. Oocytes incorporated VTG specifically and linearly for up to 24 hr. The maximum incorporation observed was 314 μg/24 hr/oocyte, using vitellogenic (3.6 mm diameter) oocytes. The effect of hormones was examined by adding insulin, growth hormone, prolactin, gonadotropins (GTH‐I, GTH‐II), thyroid hormones, testosterone, estradiol‐17β, or 17α, 20β‐dihydroxy‐4‐pregnen‐3‐one to the medium. The results indicated that insulin and thyroxine stimulated uptake of VTG by 13% and 12%, respectively. Insulin specifically stimulated VTG incorporation and did not cause any change in background uptake of insulin. The lack of an effect of gonadotropins hormones on denuded oocytes suggests that the previously observed stimulation of VTG incorporation into follicle cell‐enclosed oocytes in vivo and in vitro by GTH‐I is most likely mediated by the somatic cells of the ovarian follicle.

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