Voltage-sensing phosphatase reveals temporal regulation of TRPC3/C6/C7 channels by membrane phosphoinositides

Kyohei Itsuki, Yuko Imai, Yasushi Okamura, Kihachiro Abe, Ryuji Inoue, Masayuki X. Mori

研究成果: Contribution to journalArticle査読

16 被引用数 (Scopus)

抄録

TRPC3/C6/C7 channels, a subgroup of classical/canonical TRP channels, are activated by diacylglycerol produced via activation of phospholipase C (PLC)-coupled receptors. Recognition of the physiological importance of these channels has been steadily growing, but the mechanism by which they are regulated remains largely unknown. We recently used a membrane-resident Danio rerio voltage-sensing phosphatase (DrVSP) to study TRPC3/C6/C7 regulation and found that the channel activity was controlled by PtdIns(4,5) P2-DAG signaling in a self-limiting manner (Imai Y et al. The Journal of Physiology 2012). In this addendum, we present the advantages of using DrVSP as a molecular tool to study PtdIns(4,5)P2 regulation. DrVSP should be readily applicable for studying phosphoinositide metabolism-linked channel regulation as well as lipid dynamics. Furthermore, in comparison to other modes of self-limiting ion channel regulation, the regulation of TRPC3/C6/C7 channels seems highly susceptible to activation signal strength, which could potentially affect both open duration and the time to peak activation and inactivation. Dysfunction of such self-limiting regulation may contribute to the pathology of the cardiovascular system, gastrointestinal tract and brain, as these channels are broadly distributed and affected by numerous neurohormonal agonists.

本文言語英語
ページ(範囲)206-209
ページ数4
ジャーナルChannels
6
3
DOI
出版ステータス出版済み - 2012

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

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