Vulnerability of experimentally induced fatty liver to heat stress in rats

Hiromasa Inoue, Naomi Sameshima, Tomomi Ishida, Akiko Tsuji, Keiko Kudo, Noriaki Ikeda

研究成果: Contribution to journalArticle査読

8 被引用数 (Scopus)

抄録

Background: The aim of this study was to confirm the vulnerability of fatty liver to heat stress using fatty liver rats from the viewpoint of the induction of apoptosis. Methods: We exposed rats with and without a fatty liver to heat stress and then looked for apoptotic cells within the liver tissue using two apoptosis detection kits. We also determined the mRNA expression of heat shock protein (HSP) 70, caspase-3, bcl-2, and bax using a quantitative reverse transcription-polymerase chain reaction method. Results: Following heat stress, apoptosis was strongly visible in the fatty liver comparing with that noted in the normal liver. The expression of HSP70 was increased following heat stress in both livers, but the volume of its expression was significantly less in the fatty liver than in the normal liver. The ratio of bcl-2/bax expression tended to increase in the normal liver but decrease in the fatty liver following heat stress. Caspase-3 demonstrated no significant change following heat stress in both livers. Conclusions: The detection of apoptosis, together with changes in the mRNA expression of HSP70 and the expression ratio bcl-2/bax mRNA may indicate vulnerability of a fatty liver to heat stress and may support the hypothesis that morphologic change is induced in a fatty liver by exposure to heat stress. These results suggest that fatty liver may be more vulnerable to heat stress than normal liver.

本文言語英語
ページ(範囲)55-61
ページ数7
ジャーナルJournal of gastroenterology
41
1
DOI
出版ステータス出版済み - 1 2006

All Science Journal Classification (ASJC) codes

  • Gastroenterology

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