TY - JOUR
T1 - Wash-free detection of C-reactive protein based on third-harmonic signal measurement of magnetic markers
AU - Enpuku, Keiji
AU - Shibakura, Misako
AU - Arao, Yujiro
AU - Mizoguchi, Takako
AU - Kandori, Akihiko
AU - Hara, Misato
AU - Tsukada, Keiji
N1 - Funding Information:
This work was partly supported by the Strategic Promotion of Innovative Research and Development Program of the Japan Science and Technology Agency (Grant No. 200904001), and by a Grant-in-Aid for Scientific Research (S) (Grant No. JP15H05764) from the Japan Society for the Promotion of Science. The authors would like to thank K. Tsunashima, K. Jinno, K. Fujimoto, S. Takeuchi, and K. Fukuda for their assistance with the experiments.
Funding Information:
Acknowledgements This work was partly supported by the Strategic Promotion of Innovative Research and Development Program of the Japan Science and Technology Agency (Grant No. 200904001), and by a Grant-in-Aid for Scientific Research (S) (Grant No. JP15H05764) from the Japan Society for the Promotion of Science. The authors would like to thank K. Tsunashima, K. Jinno, K. Fujimoto, S. Takeuchi, and K. Fukuda for their assistance with the experiments.
Publisher Copyright:
© 2018 The Japan Society of Applied Physics.
PY - 2018/9
Y1 - 2018/9
N2 - We demonstrate wash-free detection of C-reactive proteins (CRPs) based on third-harmonic signal measurement of magnetic markers. In the method presented here, the CRP concentration can be detected from the decrease in the third-harmonic signal from the sample solution. The relationship between the detected signal and the CRP concentration can be modeled quantitatively using a logistic function. The quantities of CRP that were detected using the proposed method showed good correlation with those obtained using the conventional optical method with a washing process. We also demonstrate CRP detection in a hemolysis sample solution that is not optically transparent.
AB - We demonstrate wash-free detection of C-reactive proteins (CRPs) based on third-harmonic signal measurement of magnetic markers. In the method presented here, the CRP concentration can be detected from the decrease in the third-harmonic signal from the sample solution. The relationship between the detected signal and the CRP concentration can be modeled quantitatively using a logistic function. The quantities of CRP that were detected using the proposed method showed good correlation with those obtained using the conventional optical method with a washing process. We also demonstrate CRP detection in a hemolysis sample solution that is not optically transparent.
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U2 - 10.7567/JJAP.57.090309
DO - 10.7567/JJAP.57.090309
M3 - Article
AN - SCOPUS:85053412399
VL - 57
JO - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
JF - Japanese Journal of Applied Physics, Part 1: Regular Papers & Short Notes
SN - 0021-4922
IS - 9
M1 - 090309
ER -