TY - JOUR
T1 - Werner interacting protein 1 promotes binding of werner protein to template-primer DNA
AU - Kanamori, Makoto
AU - Seki, Masayuki
AU - Yoshimura, Akari
AU - Tsurimoto, Toshiki
AU - Tada, Shusuke
AU - Enomoto, Takemi
PY - 2011/8
Y1 - 2011/8
N2 - Werner interacting protein 1 (WRNIP1) that is highly conserved from Escherichia coli to human was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). Here, human WRNIP1 and WRN are shown to bind to template-primer DNA, and WRNIP1, but not WRN, requires ATP for DNA binding. Under conditions of a limiting amount of WRN, WRNIP1 facilitated binding of WRN to DNA in a dose-dependent manner. However, WRNIP1 did not stimulate the DNA helicase activity of WRN, and WRN displaced pre-bound WRNIP1 from DNA. Functional relationships between WRNIP1 and WRN will be discussed.
AB - Werner interacting protein 1 (WRNIP1) that is highly conserved from Escherichia coli to human was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). Here, human WRNIP1 and WRN are shown to bind to template-primer DNA, and WRNIP1, but not WRN, requires ATP for DNA binding. Under conditions of a limiting amount of WRN, WRNIP1 facilitated binding of WRN to DNA in a dose-dependent manner. However, WRNIP1 did not stimulate the DNA helicase activity of WRN, and WRN displaced pre-bound WRNIP1 from DNA. Functional relationships between WRNIP1 and WRN will be discussed.
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U2 - 10.1248/bpb.34.1314
DO - 10.1248/bpb.34.1314
M3 - Article
C2 - 21804224
AN - SCOPUS:80051538702
VL - 34
SP - 1314
EP - 1318
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 8
ER -