Western blotting method for the immunostaining detection of glucuronides of glycyrrhetic acid using anti-glycyrrhizin monoclonal antibody

Shao Jie Shan, Hiroyuki Tanaka, Yukihiro Shoyama

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

A method for detecting glucuronides of glycyrrhetic acid using Western blotting was investigated. Glucuronides of glycyrrhetic acid separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a glucuronides of glycyrrhetic acid-BSA conjugate. Individual spots were stained by monoclonal antibody against glycyrrhizin. Immunostaining of glucuronides of glycyrrhetic acid was more sensitive compared to other staining methods. The newly established immunostaining method can be expanded to the distribution of glucuronides of glycyrrhetic acid in the plant body.

本文言語英語
ページ(範囲)221-223
ページ数3
ジャーナルBiological and Pharmaceutical Bulletin
22
2
DOI
出版ステータス出版済み - 2 1999

All Science Journal Classification (ASJC) codes

  • 薬理学
  • 薬科学

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