Western blotting of glycyrrhetinic acid glucuronides using anti-glycyrrhizin monoclonal antibody

S. J. Shan, H. Tanaka, J. Hayashi, Y. Shoyama

研究成果: Contribution to journalArticle査読

1 被引用数 (Scopus)

抄録

A western blotting method for determining glycyrrhizin by using anti-glycyrrhizin monoclonal antibody was investigated. In this method, we found a new way to separate the glycyrrhizin molecule into two functional parts by using a simple and well-known chemical reaction. The sugar parts are oxidized to give dialdehydes, which react with amino groups on the protein that ties to the adsorbent membrane. The monoclonal antibody binds to the aglycone part of glycyrrhizin molecule. The immunocytolocalization of glycyrrhizin in Glycyrrhiza organs was investigated to indicate that the phloem contains a higher concentration of glycyrrhizin than that of the xylem. This result was confirmed by quantifying glycyrrhizin in the corresponding organs by an enzyme-linked immunosorbent assay using anti-glycyrrhizin monoclonal antibody.

本文言語英語
ページ(範囲)1491-1499
ページ数9
ジャーナルJournal of Liquid Chromatography and Related Technologies
24
10
DOI
出版ステータス出版済み - 7 26 2001

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 薬科学
  • 臨床生化学

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