Western blotting of steroidal alkaloid glycosides using monoclonal antibody against solamargine

H. Tanaka, W. Putalun, Yukihiro Shoyama

研究成果: Contribution to journalArticle査読

抄録

A method for the determination of solasodine glycosides using western blotting was investigated. Solasodine glycosides, separated by silica gel thin-layer chromatography, were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a solasodine glycoside-bovine serum albumin conjugate. Individual spots were stained with monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared with other staining methods. The newly established western blotting method is extended to the distribution of solasodine glycosides in plants.

本文言語英語
ページ(範囲)1503-1512
ページ数10
ジャーナルJournal of Liquid Chromatography and Related Technologies
22
10
DOI
出版ステータス出版済み - 1 1 1999

All Science Journal Classification (ASJC) codes

  • 分析化学
  • 生化学
  • 薬科学
  • 臨床生化学

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