Western blotting of steroidal alkaloid glycosides using monoclonal antibody against solamargine

H. Tanaka, W. Putalun, Yukihiro Shoyama

研究成果: ジャーナルへの寄稿記事

抜粋

A method for the determination of solasodine glycosides using western blotting was investigated. Solasodine glycosides, separated by silica gel thin-layer chromatography, were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a solasodine glycoside-bovine serum albumin conjugate. Individual spots were stained with monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared with other staining methods. The newly established western blotting method is extended to the distribution of solasodine glycosides in plants.

元の言語英語
ページ(範囲)1503-1512
ページ数10
ジャーナルJournal of Liquid Chromatography and Related Technologies
22
発行部数10
DOI
出版物ステータス出版済み - 1 1 1999

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Pharmaceutical Science
  • Clinical Biochemistry

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