TY - JOUR
T1 - Western blotting of steroidal alkaloid glycosides using monoclonal antibody against solamargine
AU - Tanaka, H.
AU - Putalun, W.
AU - Shoyama, Yukihiro
PY - 1999/1/1
Y1 - 1999/1/1
N2 - A method for the determination of solasodine glycosides using western blotting was investigated. Solasodine glycosides, separated by silica gel thin-layer chromatography, were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a solasodine glycoside-bovine serum albumin conjugate. Individual spots were stained with monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared with other staining methods. The newly established western blotting method is extended to the distribution of solasodine glycosides in plants.
AB - A method for the determination of solasodine glycosides using western blotting was investigated. Solasodine glycosides, separated by silica gel thin-layer chromatography, were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin, resulting in a solasodine glycoside-bovine serum albumin conjugate. Individual spots were stained with monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared with other staining methods. The newly established western blotting method is extended to the distribution of solasodine glycosides in plants.
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U2 - 10.1081/JLC-100101747
DO - 10.1081/JLC-100101747
M3 - Article
AN - SCOPUS:0032997740
VL - 22
SP - 1503
EP - 1512
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
SN - 1082-6076
IS - 10
ER -