Widespread lipoplex-mediated gene transfer to vascular endothelial cells and hemangioblasts in the vertebrate embryo

Karine Bollérot, Daisuke Sugiyama, Virginie Escriou, Rodolphe Gautier, Samuel Tozer, Daniel Scherman, Thierry Jaffredo

    研究成果: Contribution to journalArticle査読

    18 被引用数 (Scopus)

    抄録

    We report here a method that allows fast, efficient, and low-cost screening for gene function in the vascular system of the vertebrate embryo. Through intracardiac delivery of nucleic acids optimally compacted by a specific cationic lipid, we are able to induce in vivo endothelial cell-specific gain-of-function during development of the vascular network in the chick embryo. When the nucleic acids are delivered during the period of intraembryonic hematopoiesis, aortic hemangioblasts, the forerunners of the hematopoietic stem cells known to derive from the aortic endothelium, are also labeled. Similarly, we show that siRNA could be used to induce loss-of-function in vascular endothelial cells. This gene transfer technique was also applied to the mouse embryo with a high efficiency. The present method allows large-scale analysis and may represent a new and versatile tool for functional genomics.

    本文言語英語
    ページ(範囲)105-114
    ページ数10
    ジャーナルDevelopmental Dynamics
    235
    1
    DOI
    出版ステータス出版済み - 1 1 2006

    All Science Journal Classification (ASJC) codes

    • 発生生物学

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