Wild ornamental germplasm exploration and domestication based on biotechnological approaches. In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis

Alejandro S. Escandón, Ikuo Miyajima, Marisol Alderete, Juan Carlos Hagiwara, Gabriela Facciuto, Diego Mata, Silvina M. Soto

研究成果: Contribution to journalArticle査読

20 被引用数 (Scopus)

抄録

The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant. The colchicine doses tested with S. montevidiensis were: 0.0; 0.1; 0.05; 0.01 and 0.001 (24 and 48 hrs). From a total of 364 recovered plants, 4 solid tetraploid and 16 chimeras were detected. Significant differences were observed for the size of flower, leaves, and the stem diameter among the tetraploid plants and between them and the control. The tissue culture proved to be a powerful tool both to multiply the Scoparia material incorporated to our germplasm collection and to obtain new improved varieties of this beautiful genus.

本文言語英語
ページ(範囲)204-211
ページ数8
ジャーナルElectronic Journal of Biotechnology
8
2
DOI
出版ステータス出版済み - 8 15 2005

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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