Willin and Par3 cooperatively regulate epithelial apical constriction through aPKC-mediated ROCK phosphorylation

Takashi Ishiuchi, Masatoshi Takeichi

研究成果: Contribution to journalArticle査読

82 被引用数 (Scopus)

抄録

Apical-domain constriction is important for regulating epithelial morphogenesis. Epithelial cells are connected by apical junctional complexes (AJCs) that are lined with circumferential actomyosin cables. The contractility of these cables is regulated by Rho-associated kinases (ROCKs). Here, we report that Willin (a FERM-domain protein) and Par3 (a polarity-regulating protein) cooperatively regulate ROCK-dependent apical constriction. We found that Willin recruits aPKC and Par6 to the AJCs, independently of Par3. Simultaneous depletion of Willin and Par3 completely removed aPKC and Par6 from the AJCs and induced apical constriction. Induced constriction was through upregulation of the level of AJC-associated ROCKs, which was due to loss of aPKC. Our results indicate that aPKC phosphorylates ROCK and suppresses its junctional localization, thereby allowing cells to retain normally shaped apical domains. Thus, we have uncovered a Willin/Par3aPKCROCK pathway that controls epithelial apical morphology.

本文言語英語
ページ(範囲)860-866
ページ数7
ジャーナルNature Cell Biology
13
7
DOI
出版ステータス出版済み - 7 2011
外部発表はい

All Science Journal Classification (ASJC) codes

  • 細胞生物学

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