X-ray crystal structure of Escherichia coli HspQ, a protein involved in the retardation of replication initiation

Yoshito Abe, Seijiro Shioi, Shunsuke Kita, Hikaru Nakata, Katsumi Maenaka, Daisuke Kohda, Tsutomu Katayama, Tadashi Ueda

研究成果: ジャーナルへの寄稿レター

抄録

The heat shock protein HspQ (YccV) of Escherichia coli has been proposed to participate in the retardation of replication initiation in cells with the dnaA508 allele. In this study, we have determined the 2.5-Å-resolution X-ray structure of the trimer of HspQ, which is also the first structure of a member of the YccV superfamily. The acidic character of the HspQ trimer suggests an interaction surface with basic proteins. From these results, we discuss the cellular function of HspQ, including its relationship with the DnaA508 protein.

元の言語英語
ページ(範囲)3805-3816
ページ数12
ジャーナルFEBS Letters
591
発行部数22
DOI
出版物ステータス出版済み - 11 1 2017

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Crystal structure
X-Rays
X rays
Heat-Shock Proteins
Proteins
Alleles

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

これを引用

X-ray crystal structure of Escherichia coli HspQ, a protein involved in the retardation of replication initiation. / Abe, Yoshito; Shioi, Seijiro; Kita, Shunsuke; Nakata, Hikaru; Maenaka, Katsumi; Kohda, Daisuke; Katayama, Tsutomu; Ueda, Tadashi.

:: FEBS Letters, 巻 591, 番号 22, 01.11.2017, p. 3805-3816.

研究成果: ジャーナルへの寄稿レター

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AU - Abe, Yoshito

AU - Shioi, Seijiro

AU - Kita, Shunsuke

AU - Nakata, Hikaru

AU - Maenaka, Katsumi

AU - Kohda, Daisuke

AU - Katayama, Tsutomu

AU - Ueda, Tadashi

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N2 - The heat shock protein HspQ (YccV) of Escherichia coli has been proposed to participate in the retardation of replication initiation in cells with the dnaA508 allele. In this study, we have determined the 2.5-Å-resolution X-ray structure of the trimer of HspQ, which is also the first structure of a member of the YccV superfamily. The acidic character of the HspQ trimer suggests an interaction surface with basic proteins. From these results, we discuss the cellular function of HspQ, including its relationship with the DnaA508 protein.

AB - The heat shock protein HspQ (YccV) of Escherichia coli has been proposed to participate in the retardation of replication initiation in cells with the dnaA508 allele. In this study, we have determined the 2.5-Å-resolution X-ray structure of the trimer of HspQ, which is also the first structure of a member of the YccV superfamily. The acidic character of the HspQ trimer suggests an interaction surface with basic proteins. From these results, we discuss the cellular function of HspQ, including its relationship with the DnaA508 protein.

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