YB-1 is important for an early stage embryonic development: Neural tube formation and cell proliferation

Takeshi Uchiumi, Abbas Fotovati, Takakazu Sasaguri, Kohtaro Shibahara, Tatsuo Shimada, Takao Fukuda, Takanori Nakamura, Hiroto Izumi, Teruhisa Tsuzuki, Michihiko Kuwano, Kimitoshi Kohno

研究成果: Contribution to journalArticle査読

81 被引用数 (Scopus)

抄録

The eukaryotic Y-box-binding protein-1 (YB-1) is involved in the transcriptional and translational control of many biological processes, including cell proliferation. In clinical studies, the cellular level of YB-1 closely correlates with tumor growth and prognosis. To understand the role of YB-1 in vivo, especially in the developmental process, we generated YB-1 knock-out mice, which are embryonic lethal and exhibit exencephaly associated with abnormal patterns of cell proliferation within the neuroepithelium. β-Actin expression and F-actin formation were reduced in the YB-1 null embryo and YB-1-/- mouse embryonic fibroblasts, suggesting that the neural tube defect is caused by abnormal cell morphology and actin assembly within the neuroepithelium. Fibroblasts derived from YB-1-/- embryos demonstrated reduced growth and cell density. A colony formation assay showed that YB-1-/- mouse embryonic fibroblasts failed to undergo morphological transformation and remained contact-inhibited in culture. These results demonstrate that YB-1 is involved in early mouse development, including neural tube closure and cell proliferation.

本文言語英語
ページ(範囲)40440-40449
ページ数10
ジャーナルJournal of Biological Chemistry
281
52
DOI
出版ステータス出版済み - 12 29 2006

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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