Yeast-based recombineering of DNA fragments into plant transformation vectors by one-step transformation

Yukio Nagano, Syoko Takao, Takahiro Kudo, Ei'ichi Iizasa, Toyoaki Anai

研究成果: Contribution to journalArticle査読

11 被引用数 (Scopus)

抄録

T-DNA binary vectors are often used in plant transformation experiments. Because they are usually very large and have few restriction sites suitable for DNA ligation reactions, cloning DNA fragments into these vectors is difficult. We provide herein an alternative to cloning DNA fragments into very large vectors. Our yeast-based recombineering method enables DNA fragments to be cloned into certain types of T-DNA binary vectors by one-step transformation without the requirement of specific recombination sites or precisely positioned restriction ends, thus making the cloning process more flexible. Moreover, this method is inexpensive and is applicable to multifragment cloning.

本文言語英語
ページ(範囲)2111-2117
ページ数7
ジャーナルPlant Cell Reports
26
12
DOI
出版ステータス出版済み - 12 2007
外部発表はい

All Science Journal Classification (ASJC) codes

  • 農業および作物学
  • 植物科学

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