TY - JOUR
T1 - Yolk of a "scanty vitellin" mutant, vit, of Bombyx mori is lacking in vitellin and 30 kDa proteins
AU - Fujikawa, Kazuhiro
AU - Kawaguchi, Yutaka
AU - Banno, Yutaka
AU - Koga, Katsumi
AU - Doira, Hiroshi
N1 - Funding Information:
Fig. 2. Changesi n concentrationo f haemolymphp rotein of femaled uring pupal-adultd evelopmentO. pen circles, normal:c losed circles, vit. For abscissas ee Fig. 1. Verti-cal bars, standardd eviation( n = 5): Acknowledgements-The study was supportedi n part by a Grant-in-Aid for Scientific Researchf rom the Ministry of Education, Science and Culture of Japan.
PY - 1995
Y1 - 1995
N2 - A novel mutant named "scanty vitellin" (vit) of Bombyx mori, induced by N-methyl-N-nitrosourea, was analyzed for yolk proteins by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The ovary of vit/vit pupae was lacking in vitellin and the 30 kDa proteins that are yolk constituents synthesized outside the ovary. However, the vit/vit ovary had a normal amount of the egg-specific protein, a yolk component synthesized by the ovarian follicular epithelial cells. On the other hand, vitellogenin and the 30 kDa proteins, which are the precursors of yolk proteins, were abundant in the haemolymph of vit/vit female pupae. These results indicate that the mutant gene interferes with the uptake of the extraovarian yolk precursors into the oocyte.
AB - A novel mutant named "scanty vitellin" (vit) of Bombyx mori, induced by N-methyl-N-nitrosourea, was analyzed for yolk proteins by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The ovary of vit/vit pupae was lacking in vitellin and the 30 kDa proteins that are yolk constituents synthesized outside the ovary. However, the vit/vit ovary had a normal amount of the egg-specific protein, a yolk component synthesized by the ovarian follicular epithelial cells. On the other hand, vitellogenin and the 30 kDa proteins, which are the precursors of yolk proteins, were abundant in the haemolymph of vit/vit female pupae. These results indicate that the mutant gene interferes with the uptake of the extraovarian yolk precursors into the oocyte.
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U2 - 10.1016/0300-9629(95)02031-4
DO - 10.1016/0300-9629(95)02031-4
M3 - Article
AN - SCOPUS:0029620357
VL - 112
SP - 585
EP - 589
JO - Comparative Biochemistry and Physiology -- Part A: Physiology
JF - Comparative Biochemistry and Physiology -- Part A: Physiology
SN - 0300-9629
IS - 3-4
ER -