ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model

Yasuhiro Yoshida, Toshiyuki Tsunoda, Yasuo Takashima, Takahiro Fujimoto, Keiko Doi, Takehiko Sasazuki, Masahide Kuroki, Akinori Iwasaki, Senji Shirasawa

研究成果: Contribution to journalArticle査読

15 被引用数 (Scopus)

抄録

ZFAT, originally identified as a susceptibility gene for autoimmune thyroid disease, encodes a transcriptional regulator with one AT-hook and 18 C2H2-type zinc-finger domains. It is highly conserved among species. Here, we demonstrate that ZFAT is clearly expressed in human umbilical vein endothelial cells (HUVECs). Furthermore, we show that endothelial cell assembly and the branch point formation of capillary-like structures in HUVECs is impaired by the reduction of ZFAT expression through the use of ZFAT-miRNAs, whereas differences in cell proliferation or apoptotic features were not observed after the reduction in ZFAT expression. These results suggest that ZFAT may have critical roles in the capillary-like network formation that is involved in vascular remodeling. Elucidating the ZFAT-mediated transcriptional network will lead to a better understanding of the molecular mechanisms of angiogenesis.

本文言語英語
ページ(範囲)541-550
ページ数10
ジャーナルCellular and Molecular Biology Letters
15
4
DOI
出版ステータス出版済み - 2010

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 細胞生物学

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